Plasmid-DNA pUC19

The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchange chromatography. It is available in supercoiled form (ccc-form >95%) and, as a high-purity DNA (OD_260/280 > 1,70), it is ideal for different applications:

pUC19 is a standard high-copy cloning vector for E. coli recombinants. The position of the Multi Cloning Site (MCS), which is inserted in frame into the LacZ-gene, enables a blue-white selection of insert containing plasmid DNA by α-complementation. The copy number of plasmids per cell depends on the temperature and is approx. 70-80 at 37 °C and over 200 at 42 °C.