Proteinase K, 250 mg, 1 x 250 mg
Art.-Nr. 7528.5
€ 240,43
lieferbar
1
Auf den Merkzettel
Proteinase K, 250 mg, 1 x 250 mg
Produktbeschreibung
P-Sätze: P210 P260 P280 P302+P352 P305+P351+P338 P308+P311
H-Sätze: H225-H290-H302+H312+H332-H318-H336-H370
Gefahrenklasse: 3 (8) II
HS-Code: 35079090
Marke: Carl Roth
Proteinase K, lyophil., min. 30 mAnson U/mg, Storage temp. +4 °C, CAS No. [39450-01-6], EG-Nr. 254-457-8
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Gebruiksinstructies
Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Proteinase K ≥30 mAnson U/mg, lyophilized
A non-specific endopeptidase with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.
Gebruiksinstructies
Working solution: 50 µg/ml
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
| Activity (Hemoglobin, 37 °C) | ≥30 U/mg |
| DNases, RNases | not detected |
| Endonucleases | not detected |
Das könnte Ihnen auch gefallen
5-Ethynyl-1-methyl-1H-1,2,3-triazole, 1g
5-Ethynyl-1-methyl-1H-1,2,3-triazole, 1g
Art.-Nr. F983357-1G
€ 1 509,20
Gallium tris(trifluoromethanesulfonate), 95%, 250mg
Gallium tris(trifluoromethanesulfonate), 95%, 250mg
Art.-Nr. F987360-250MG
€ 19,80
1,6-Heptadien-4-ol, 4-(2-propen-1-yl)-, 95+%, 5g
1,6-Heptadien-4-ol, 4-(2-propen-1-yl)-, 95+%, 5g
Art.-Nr. F601302-5G
€ 115,50
![4-Chloro-2-(trifluoromethyl)pyrido[3,4-d]pyrimidine, 97.0%, 1g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4863432811.png "4-Chloro-2-(trifluoromethyl)pyrido[3,4-d]pyrimidine, 97.0%, 1g")
Ethyl 3-(5-methoxy-1H-indol-3-yl)propanoate, 97+%, 1g
Ethyl 3-(5-methoxy-1H-indol-3-yl)propanoate, 97+%, 1g
Art.-Nr. F626950-1G
€ 353,10
![4,7-DIAZASPIRO[2.5]OCTANE-5,8-DIONE, 95.0%, 5g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4863525987.png "4,7-DIAZASPIRO[2.5]OCTANE-5,8-DIONE, 95.0%, 5g")
4,7-DIAZASPIRO[2.5]OCTANE-5,8-DIONE, 95.0%, 5g
4,7-DIAZASPIRO[2.5]OCTANE-5,8-DIONE, 95.0%, 5g
Art.-Nr. F331971-5G
€ 616,00
Powered by Lightspeed
Preise anzeigen in:EUR
