ROTI®Cell Iscoves MDM
Art.-Nr. 9033.1
€ 28,20
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ROTI®Cell Iscoves MDM
Produktbeschreibung
HS-Code: 38210000
Marke: Carl Roth
ROTI®CELL Iscove´s MDM, sterile, with glutamine, pyruvate and, HEPES, ready-to-use, CELLPURE®, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +2 to +8 °C
Steriel
Iscove’s Modified Dulbecco’s Medium is based on DMEM High Glucose, containing selenium, HEPES, and, instead of iron, potassium (as potassium nitrate) besides additional amino acids and vitamins. It is well suited for culturing of a wide range of mammalian cell lines and primary cells, including B- and T lymphocytes as well as hybridoma cells.
When working without FBS, growth factors, hormones and lipids, which are essential for cell growth, must also be added to the medium.
ROTI®Cell Iscove's MDM CELLPURE® ready-to-use, sterile, with glutamine, with pyruvate, with HEPES
This modification of IMDM contains L-glutamine, pyruvate and 25 mM HEPES.
Technische informatie
| Toepassing | Cell culture |
| Gebruikstip | Store at +2 °C to +8 °C protected from light. Once opened, use within 6-8 weeks. |
| Aanvullende informatie | The detailed composition can be found in the specification. |
The buffer system in cell culture media
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
ROTI®Cell Media for Cell Culture - Composition
| Medium | Art. No. | Glucose g/l |
Glutamine (mM) |
HEPES (mM) |
Sodium Pyruvate (mM) |
NaHCO3 (mM) |
Phenol Red |
Salts | Note |
| Dulbecco’s Modified Eagle Media | |||||||||
| ROTI®Cell DMEM High Glucose | 9005.1 | 4.5 | 4 | X | 1 | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9006.1 | 4.5 | 4 | X | X | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9007.1 | 4.5 | 4 stab. | X | 1 | 44 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell DMEM High Glucose | 1TE9.1 | 4,5 | 4 stab. | 25 | X | 44 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell DMEM High Glucose | 9010.1 | 4.5 | X | X | 1 | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9019.1 | 4.5 | X | X | X | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 1TE8.1 | 4.5 | X | X | X | 44 | X | X | Without phenol red. |
| ROTI®Cell DMEM Low Glucose | 1TE7.1 | 1.0 | 4 stab. | X | 1 | 44 | yes | With temperature stabile glutamine. | |
| ROTI®Cell DMEM Low Glucose | 9027.1 | 1.0 | X | X | 1 | 44 | yes | X | |
| DMEM : F12 | |||||||||
| ROTI®Cell DMEM : F12 | 1TE5.1 | 3.151 | X | 15 | 0,5 | 14.3 | yes | X | |
| ROTI®Cell DMEM : F12 | 1YTP.1 | 3.151 | 2.5 | 15 | 0,5 | 14.3 | yes | X | |
| Iscove’s Modfied Dulbecco Media | |||||||||
| ROTI®Cell Iscove’s MDM | 9033.1 | 4.5 | 4 | 25 | 1 | 36 | yes | X | Additional amino acids and selene. |
| Minimum Essential Media | |||||||||
| ROTI®Cell Eagle’s MEM / Earle’s | 9044.1 | 1 | 2 | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell Eagle’s MEM / Earle’s | 1TE6.1 | 1 | 2 stab. | X | X | 26.2 | yes | Earle’s | With temperature stabile glutamine. |
| ROTI®Cell Eagle’s MEM / Earle’s | 9047.1 | 1 | X | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell Eagle’s MEM-Alpha | 9058.1 | 1 | X | X | 1 | 26.2 | yes | Earle’s | Plus cobalamin, ascorbic acid, NEAA*, lipoic acid, biotin. |
| ROTI®Cell Eagle’s MEM-Alpha | 1TEA.1 | 1 | X | X | 1 | 26.2 | yes | Earle’s | Plus cobalamin, ascorbic acid, NEAA*, lipoic acid, biotin, ribonucleosides and desoxyribonucleosides. |
| RPMI Media | |||||||||
| ROTI®Cell RPMI 1640 | 9085.1 | 2 | 2 | X | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9086.1 | 2 | 2 | 25 | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9091.1 | 2 | 2 stab. | X | X | 23.8 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell RPMI 1640 | 9099.1 | 2 | X | X | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9104.1 | 2 | X | X | X | 23.8 | X | X | |
| Special Media | |||||||||
| ROTI®Cell Ham’s F12 | 9108.1 | 1.8 | 1 | X | 1 | 14 | yes | X | |
| ROTI®Cell Leibovitz’s L15 | 9109.1 | 0.9 galact. | X | X | 5 | X | yes | X | With galactose instead of glucose. Without sodium bicarbonate. |
| ROTI®Cell McCoy’s 5A | 9111.1 | 3 | 1.5 | X | X | 26.2 | yes | X | |
| ROTI®Cell Medium 199 / Earle’s | 9112.1 | 1 | 0.7 | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell TC100 | 9114.2 | 1 | 4,1 | X | X | 4,2 | X | X | With Tryptose Broth. |
| * NEAA: Non-essential amino acids | |||||||||
ROTI®Cell Media for Cell Culture - Application
| Medium | Art. No. | CO2 (%) | Serum | Field of application | Successfully cultured cells |
| Dulbecco’s Modified Eagle Media | |||||
| ROTI®Cell DMEM | 9005.1 | 8,5 | recommend | Frequently used universal medium with up to 4-fold higher concentrations of amino acids and vitamines. | Primary fibroblasts, neurons, glial cells, smooth muscle cells, epithelial cells, tumor cells, hybridome culturesHUVEC, HeLa, HEK 293, PC-12NIH 3T3, 3T6, A-431, A9 L, BALB 3T3, BSC40, COS-1, COS-7, EB, L6, PK1 |
| 9006.1 | |||||
| 9007.1 | |||||
| 1TE9.1 | |||||
| 9010.1 | |||||
| 9019.1 | |||||
| 1TE8.1 | |||||
| 1TE7.1 | |||||
| 9027.1 | |||||
| DMEM : F12 | |||||
| ROTI®Cell DMEM : F12 | 1TE5.1 | 5,5 | optional | Highly complex medium for versatile use.Basis of many serum-free media. | Primary fibroblasts, neurons, HU-VECs, smooth muscle cellsHEK-293, HeLa, Cos-7, PC-12 |
| 1YTP.1 | |||||
| Iscove’s Modfied Dulbecco Media | |||||
| ROTI®Cell Iscove’s MDM | 9033.1 | 7,0 | optional | For fast proliferating cells, high cell densities and mass cell cultures. | Bone marrow cells, lymphocytes, macrophages, Hybridoma culturesKG-1, COS-7, Jurkat |
| Minimum Essential Media | |||||
| ROTI®Cell Eagle’s MEM / Earle’s | 9044.1 | 5,0 | recommend | For cells with special nutritionalrequirements. | Numerous suspension and adherent cells, mammalian fibroblastsHek293, BS-C-1, CV-1, Hep G2, JEG-3, L-929, McCoy, MDBK, MDCK, PK(15), PtK1, RK13, Vero |
| ROTI®Cell Eagle’s MEM / Earle’s | 1TE6.1 | 5,0 | recommend | ||
| ROTI®Cell Eagle’s MEM / Earle’s | 9047.1 | 5,0 | |||
| ROTI®Cell Eagle’s MEM-Alpha | 9058.1 | 5,0 | Additionally suitable for selection of transfected DG44 and other AHFR-negative cells. | BHK-21, BT, Caco-2, CRFK, D-17, HeLa, HT-1080, XC | |
| ROTI®Cell Eagle’s MEM-Alpha | 1TEA.1 | 5,0 | recommend | For cells with special nutritionalrequirements. | |
| RPMI Media | |||||
| ROTI®Cell RPMI 1640 | 9085.1 | 4,5 | recommend | For single cell lines also suitable without serum. | Lymphocytes, leukemia cells, hybridoma cultures, astrocytes, carcinoma cellsDaudi, H9, HCT-15, HL-60, IM-9, Jurkat, K-562, Raji, MCF-7, HeLa, PC12, PBMC |
| 9086.1 | |||||
| 9091.1 | |||||
| 9099.1 | |||||
| 9104.1 | |||||
| Special Media | |||||
| ROTI®Cell Ham’s F12 | 9108.1 | 2,5 | optional | Widely used, highly complex medium. | Specifically for mouse CHO cells, lung cells and L cellsCHO-K1 |
| ROTI®Cell Leibovitz’s L15 | 9109.1 | 0,0* | recommend | Suitable for fast growing cells in relatively high density. | Tumor and embryonic cellsHeLa, Hep-2, SW-13 |
| ROTI®Cell McCoy’s 5A | 9111.1 | 5,0 | recommend | Standard medium for clonal growth of hepatoma cell linesUsed in virus production. | Hepatoma cell lines, other permanent cell lines and also primary cellsHT-29, Jensen |
| ROTI®Cell Medium 199 / Earle’s | 9112.1 | 5,0 | optional | Highly complex medium versatile. | Especially suitable for non-transformed cellsLLC-PK1, LLC-WRC 256 |
| ROTI®Cell TC100 | 9114.2 | 0,0* | recommend | Insect medium for the culture of a variety of Lepidopter species cells. | Sf9, Sf21 |
| * Cultivation outside the CO2 incubator. | |||||
| Glucose | 4,5 g/l |
| L-Glutamine | complies |
| HEPES | 25 mM |
| pH value | 7.0-7.6 |
| Osmolality | 275-305 mOsm/kg |
| Endotoxins | ≤1,0 EU/ml |
| Sterility | complies |
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