ROTI®Blue fast, 1 l, plástico
REF 4829.2
€ 408,36
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ROTI®Blue fast, 1 l, plástico
Información del producto
Número CAS: 632-69-9
fórmulas químicas: C20H2Cl4I4Na2O5
Código hs: 38229000
Marca: Carl Roth
ROTI®Blue quick, 1x solution, ready-to-use, staining solution for protein gels, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C, UN-Nr. 1760
ROTI®Blue quick 1x conc., ready-to-use
ROTI®Blue quick provides a new, very fast staining method for proteins in polyacrylamide gels. Due to the modern formulation, the dye binds highly specifically to proteins, but not to the gel matrix, hence eliminating the need to destain the gel. First bands are clearly visible within 3 minutes!
- Particularly fast, specific staining
- First bands are visible within 3 mins.
- No background - no destaining necessary
- Very simple application without heating, methanol, or acetic acid
- Stabilised for a longer shelf life
Figure:
SDS-PAGE with 3 µl Roti®-Mark Standard and samples.
Staining duration: 20 mins.
SDS-PAGE with 3 µl Roti®-Mark Standard and samples.
Staining duration: 20 mins.
Gebruiksinstructies
The following protein amounts / band can be detected in the specified time (minutes):
from 1 µg (3 min.)
from 100 µg (15 min.)
from 10 ng (60 min.)
The sensitivity of staining equals that of ROTI®Blue (Art. No. A152), so bands of 10 ng protein can be detected. ROTI®Blue quick is, therefore, approx. 5times more sensitive than standard Coomassie® staining solutions. ROTI®Blue quick staining is also fully compatible with usual down-stream applications like, membrane transfer & Western blotting, MS analysis (incl. MALDI) and protein gel elution.
Technische informatie
| Sensitiviteit | 10 |
| Toepassing | Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics. |
Suitability of Protein Stainings
| Product | Sensitivity | Rapid Gel Analysis |
Gel Drying |
Elution/ Transfer |
2D- Gels |
MS- Analysis |
| ROTI®Black P | 1 ng/mm2 | - | +++ | - | +++ | - |
| ROTI®Blue | 10 ng/mm2 | - | +++ | +++ | - | +++ |
| ROTI®Blue quick | 10 ng/mm2 | +++ | +++ | ++ | - | ++ |
| ROTI®Blau R | 50 ng/mm2 | +++ | + | - | - | - |
| +++: recommended +: suitable -: not recommended | ||||||
Summary Staining of Protein Gels
| Product | Sensitivity | Description |
| ROTI®Black P | 1 ng/mm2 | Silver staining kit. Highly sensitive. Simple, fast and reproducible. |
| ROTI®Blue | 10 ng/mm2 | Coomassie®-solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |
| ROTI®Blue quick | 10 ng/mm2 | Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics. |
| ROTIPHORESE®Blau R | 50 ng/mm2 | Coomassie® staining solution concentrate for rapid staining. |
| Coomassie® Blue G250 (C.I. 42655) | 60 ng/mm2 | Dye for performing Coomassie® staining. ODmax = 595 |
| Coomassie® Blue R250 (C.I. 42660) | 50 ng/mm2 | Dye for performing Coomassie staining. ODmax = 550 |
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