ROTI®Garose-His/Ni NTA-Cartuchos, 1 x 5 ml

For the isolation of His-tagged proteins under reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI^®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.

The matrix of ROTI^®Garose-His/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidine tags with good specificity and very high affinity, making the Ni²⁺ charged matrix the first choice for all standard applications. The tetradentate NTA cross-linker binds his-tagged proteins very efficiently, leading to high recovery rates with minimized nickel bleeding into the eluate.

ROTI^®Garose-His/Ni NTA-Beads may be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM glutathion, ≤10 mM DTT, ≤10 mM DTE, ≤20 mM β-mercaptoethanol und ≤0,3 % SDS).

Prepacked cartridges with Nickel charged NTA-agarose bead matrix for medium pressure affinity chromatography (MPLC) under reducing conditions.
Immobilized metal ion affinity chromatography (IMAC) under medium pressure is the prefect method to obtain high yield of very pure proteins in minimum time. ROTI^®Garose-His/Ni NTA-Cartridges offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates in reducing buffer solutions.
Matrix in 15 % ethanol.

ÄKTA™FPLC™ and for sample application via peristaltic pump or syringe.

ROTI^®Garose His/Ni NTA-Cartridges are prepacked and may be used directly.

Column material made from polypropylene and polyethylene (frit), Inner diameter 1,6 cm, height 2,5 cm, inlet port 10-32 (1/16″) female, outlet port 10-32 (1/16″) male.