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ROTI®Pol Hot-TaqS, 200 µl, 5 x 40 µl

ROTI®Pol Hot-TaqS, 200 µl, 5 x 40 µl

REF 9245.2
€ 401,46
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ROTI®Pol Hot-TaqS, 200 µl, 5 x 40 µl
Información del producto
Código hs: 38229000
Marca: Carl Roth
ROTI®Pol Hot-TaqS (5 x 40 µl), 5 U/µl, Storage temp. -20 °C

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.


ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.



ROTI®Pol Hot-TaqS 5 U/μl

Hot start version of the recombinant heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer, plus additional 10x concentrated PCR reaction buffer and 10x concentrated PCR reaction buffer with red gel loading dye. ROTI®Pol Hot-TaqS is recommended for use in highly specific PCR applications.

This polymerase set ROTI®Pol Hot-TaqS is outstandingly suitable for all Taq-based cycling protocols, in which particularly specific amplification is the main focus - as being performed in, for instance, prior to cloning or sequencing processes, in cycle sequencing, and in similar assays. In combination with our unique buffers, the Hot-TaqS polymerase delivers highly specific PCR amplification of good yield with a wide range of PCR templates. The antibody-mediated blocking of the DNA polymerase is released only at the initial denaturation step, hence resulting in highly specific amplification of the target sequence without production of unwanted side products caused by unspecific primer annealing.

ROTI®Pol Hot-TaqS is able to amplify PCR products up to 3 kb with genomic DNA and up to at least 5 kb in size with Lambda DNA and is appropriate for use in the amplification of DNA from genomic, viral, and plasmid templates. The Hot-TaqS DNA polymerase included in the set possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

RotiPol-Sensitivitaetsassay
Figure: Sensitivity assay using ROTI®Pol TaqS, TaqHY, Hot-TaqS and Hot-TaqHY, 1 U/reaction each (20 μl). 300 bp β-Actin fragment, 40 cycles. Template: 10 to 2000 pg human gDNA. Gel loading 10 μl each.
M: 100 bp-DNA Ladder extended. NTC: no template control.

Filled in colour coded tubes, the set contains the DNA polymerase and two 10x concentrated reaction buffers with 20 mM MgCl2, one of which has been specially designed for direct gel loading following the PCR reaction. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The use of the colourless PCR reaction buffer is adequate for all general PCR applications and is particularly recommended when direct fluorescence or absorbance readings are required.


De set bevat

Hot-TaqS polymerase in storage buffer containing 50 % glycerol, PCR buffer (10x) incl. 20 mM MgCl2, PCR buffer red (10x) with 20 mM MgCl2 and 0,1 % cresol red.
Colour coded tubes.
Contents of this set may not be bought separately.



Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Toepassing Particularly sequence specific standard PCR
Amplicon-einden 3'dA
Polymerase Hot start Taq polymerase
Reactiebuffer colourless + red (ready to load)
Toepassing Particularly sequence specific standard PCR

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

• Oxidoreductases (catalyse redox reactions)

• Transferases (transfer functional groups among substrates)

• Hydrolases (cleave bonds via addition of water)

• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

• Isomerases (transform chemical isomers)

• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Purity (enzyme) ≥98 %
Enzyme activity 5 U/µl
Endonuclease activity none detected
Exonuclease activity none detected
Suitability for PCR (400 bp) complies
Suitability for PCR (3 kb) complies
Suitability for PCR (5 kb) complies
Selective specificity in PCR complies
Hot start functionality complies
Stability (in storage buffer) complies
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