ROTI®Pol TaqS Red-Mix (2x), 10 ml, 10 x 1 ml

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI^®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI^®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

Optimized pre-mixed 2x PCR solution containing the recombinant TaqS DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl₂, and all other components required for PCR (w/o primers and template DNA), plus components for direct gel electrophoresis. ROTI^®Pol TaqS Red-Mix (2x) is recommended for use in all standard PCR applications immediately followed by gel electrophoresis.

PCR assaying with ROTI^®Pol TaqS Red-Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI^®Pol TaqS Red-Mix (2x) can be applied to all standard Taq-based cycling protocols and is, therefore, the optimal choice for high throughput PCR as being performed in, for instance, analysis of cloning efficiency or colony screening, for routine screening processes, and for student's courses.

Due to the optimized composition of the master mix, the TaqS polymerase delivers specific PCR amplification of good yield with a wide range of PCR templates. ROTI^®Pol TaqS Red-Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The TaqS polymerase included in the master mix possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

Composition:
2 or 10 vials, respectively, with 1,25 ml each of ROTI^®Pol TaqS Red-Mix (2x) containing TaqS polymerase, 0,4 mM each dNTP, and 4 mM MgCl₂ in 2x reaction buffer with 0,02 % cresol red.