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ROTI®Prep gDNA Mini 2.0, 50 preparaciones

ROTI®Prep gDNA Mini 2.0, 50 preparaciones

REF 1YTK.2
€ 247,33
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ROTI®Prep gDNA Mini 2.0, 50 preparaciones
Información del producto
Número CAS: 7732-18-5
fórmulas químicas: H2O
Código hs: 38221900
Marca: Carl Roth
ROTI®Prep gDNA Mini 2.0, 50 pcs, for molecular biology, Density (D) ~1 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 3316


ROTI®Prep gDNA Mini 2.0 for molecular biology

ROTI®Prep gDNA MINI 2.0 was developed to isolate genomic DNA from a wide variety of starting materials.
The isolation is based on a new procedure and combines sample lysis with the subsequent binding of the DNA to the surface of a column membrane. After several washing and centrifugation steps, the DNA is eluted from the membrane using an elution buffer. The extraction procedure in this kit is optimised to achieve maximum yield.

  • For DNA extraction from bacteria, fungi and eukaryotic cell, blood and tissue samples.
  • Preparation in the well-established mini-column system
  • Fast, simple and reliable
  • Extraction time of approx. 8 min (without lysis step)
  • Yield, depending on the starting material, of up to 65 µg gDNA

Mogelijk uitgangsmateriaal:
  • Microbial cell cultures (max. 5x1010)
  • Fungi and eukaryotic cell cultures (max. 5x106)
  • Rodent tail (0.5-1 cm)
  • Oral mucosal swabs
  • Tissue samples
  • Blood (200-400 µl)

The kit contains:

Lysis Buffer LSV, Lysis Buffer LSN, Binding Buffer BR, Binding Buffer BSN, Proteinase K, RNase A (10 mg/ml), Washing Buffer WSO, Washing Buffer WSH (conc.), Washing Buffer WST (conc.), Elution Buffer EB, Centrifugation columns, Collection tubes, Elution tubes, detailed instructions.
Ethanol 99.8 % (p.a., order no. 9065), RNase free water (order no. 1HPE.1), TE buffer (order no. 1052.1) and PBS (order no. 1058.1) are not included in the kit and should be ordered separately if required.



Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Gebruikstip For DNA extraction from harder or more complex samples (bacteria, fungi) we recommend preparing the sample with a suitable ROTI®SampleLyse tube (e.g. Art.No. 1YKE.1). For DNA extraction from plant tissue, we recommend ROTI®Prep Plant DNA (Art. No. 20H4.1).
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