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ROTIPHORESE®Gel A, 1 l

ROTIPHORESE®Gel A, 1 l

REF 3037.1
€ 73,08
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ROTIPHORESE®Gel A, 1 l
Información del producto
P-frases: P305+P351+P338 P337+P313 P406
H-frases: H290-H319
Nivel de riesgo: 8 III
Código hs: 38229000
Marca: Carl Roth
ROTIPHORESE® Gel A, 30 % acrylamide stock solution, gas stabilised, Density (D) 1,023 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

Ready-to-use, gas-stabilized acrylamide ready-to-use solution. The ROTIPHORESE® gel solution is very easy to use and enables reproducible and high-resolution gel electrophoresis.


ROTIPHORESE® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers.
The ROTIPHORESE® acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas.
In order to ensure a high lot-to-lot consistency of our ROTIPHORESE® solutions, each batch is subjected to an electrophoretic test.


Mechanisme

PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).
With the ROTIPHORESE® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).


PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).
With the ROTIPHORESE® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).

For detailed instructions regarding gel preparation with ROTIPHORESE® Gel A, A-40 and B please see user manual and technical information brochure “PAGE Instructions”.



ROTIPHORESE®Gel A ROTIPHORESE® 30 %, gas-stabilized
Gebruiksinstructies

Ready-to-use, stabilised 30 % acrylamide stock solution. In combination with ROTIPHORESE®Gel B (2 % bisacrylamide stock solution, Art. No. 3039) gels with up to 20 % total acrylamide concentration can be produced.


For detailed instructions regarding gel preparation with ROTIPHORESE®Gel A, A-40 and B please see our Technical Information Brochure “PAGE Instructions”.



Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.

Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.


The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.


ROTIPHORESE®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE® ready-to-use solutions can be used directly.


Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions

Analysis of Gel solution Art. No.
Proteins/peptides >15 kDa, DNA fragments Gel 30 (37,5:1) 3029.1
Gel 40 (37,5:1) T802.1
Proteins/peptides <30 kDa and DNA oligonucleotides Gel 40 (29:1) A515.1
DNA sequences (manual sequencing) ROTIPHORESE®DNA sequencing system A431.1
Gel 40 (19:1) 3030.1
DNA sequences (short-run automat. sequencing) NF solution 40 % (19:1) A516.1
DNA sequences (long-run automat. sequencing) NF solution 30 % (29:1) A124.1
NF solution 40 % (29:1) A121.1

Filled under protective gas., Each batch is functionally tested for its suitability in electrophoresis., Note: Product may crystallise. It can be liquefied by heating in a water bath to max. 40 °C.
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