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monoclonal de ratón antidesmocolina 1, Dsc1-U100, 0,2 ml

monoclonal de ratón antidesmocolina 1, Dsc1-U100, 0,2 ml

REF 1NN6.1
€ 110,31
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monoclonal de ratón antidesmocolina 1, Dsc1-U100, 0,2 ml
Información del producto
P-frases: P280 P302+P352 P304+P340 P305+P351+P338 P312
H-frases: H315-H319-H335
Número CAS: 14933-08-05
fórmulas químicas: C17H37NO3S
Código hs: 30021500
Marca: PROGEN
anti-Desmocollin 1 mouse, monoclonal, Dsc1-U100, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-Desmocollin 1 mouse monoclonal, Dsc1-U100 50 μg/ml, monoclonal, affinity purified

Mouse monoclonal antibody localizes Dsc1 present in suprabasal layers of interfollicular epidermis, in specific cell layers (e.g. in hair follicle root sheath) and in hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).

Reacts with 111 kDa (form 'a') and 103 kDa (form 'b') polypeptides (desmocollin 1 splice forms, both at pI 5.2) from human epidermal desmosomes.

Reactivity on cultured cell lines: positive on primary keratinocytes, however, restricted to local piles of differentiated cells. All simple epithelia are negative.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: Dsc1-U100
Immunogen: synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC)
UniprotID: Q08554 (Human)
Synonym: desmocollin-1, DSC1, cadherin family member 1, desmosomal glycoprotein 2/3, DG2/DG3, CDHF1
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IHC, WB
Reactivity: human, mouse, rat


Toepassing

Immunohistochemistry (IHC) - frozen. 1:50-1:100 (0.5-1 μg/ml, preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended; without detergent pretreatment a cytoplasmic component might cross-react)
Immunohistochemistry (IHC) - paraffin. 1:50-1:100 (0.5-1 μg/ml, microwave treatment recommended; a slight crossreactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step)
Western Blot (WB): Assay dependent


1NN6_01
IHC of rat skin with anti-desmocollin 1 (1:50, courtesy of J.Heß, University Hospital Heidelberg)
1NN6_02
anti-Desmocollin 1 mouse monoclonal, Dsc1-U100, liquid, purified
1NN6_03
IHC of mouse skin with anti-desmocollin 1 (1:50, courtesy of J.Heß, University Hospital Heidelberg)


Geen medisch hulpmiddel / Geen ivD-product
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