monoclonal de ratón anti-etiqueta GST, F50-3D12.2, 100 µg
REF 1NN8.2
€ 454,37
Disponible
1
Guardar este producto para más tarde
monoclonal de ratón anti-etiqueta GST, F50-3D12.2, 100 µg
Información del producto
P-frases: P280 P302+P352 P304+P340 P305+P351+P338
H-frases: H315-H319-H335
Número CAS: 14933-09-06
fórmulas químicas: C19H41NO3S
Código hs: 30021500
Marca: PROGEN
anti-GST-tag mouse, monoclonal, F50-3D12.2, after dissolv. in 400 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-GST-tag mouse monoclonal, F50-3D12.2 lyophilized, monoclonal, affinity purified
The monoclonal F50-3D12.2 antibody recognizes the GST-tag (Glutathione S-transferase). The GST-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: F50-3D12.2Immunogen: not available or unknown
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: WB
Reactivity: GST
Toepassing
Western Blot (WB): 1:500 (0.5 μg/ml)
Western blot analysis of E. coli lysate containing GST-tagged protein with anti-GST-tag antibody. Western blot analysis was performed on 100 ng or 25 ng of E. coli lysate containing GST-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-GST-tag mouse monoclonal, F50-3D12.2 (Cat. No. 910GSTL) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
Productos relacionados
3-Pyridin-3-yl-1H-pyrazol-5-amine, 95.0%, 1g
3-Pyridin-3-yl-1H-pyrazol-5-amine, 95.0%, 1g
REF F064906-1G
€ 160,60
![6-Bromo-3-chloro-benzo[b]thiophene-2-carboxylic acid, 95.0%, 5g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4868287345.png "6-Bromo-3-chloro-benzo[b]thiophene-2-carboxylic acid, 95.0%, 5g")
![6-Bromoimidazo[1,2-a]pyrazin-2-amine, 95.0%, 100mg](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4868271143.png "6-Bromoimidazo[1,2-a]pyrazin-2-amine, 95.0%, 100mg")
![7H-PYRROLO[2,3-D]PYRIMIDINE-4-CARBONITRILE, 97.0%, 250mg](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4782657747.png "7H-PYRROLO[2,3-D]PYRIMIDINE-4-CARBONITRILE, 97.0%, 250mg")
![1-(2-ETHOXYETHYL)-2-(4-METHYL-1,4-DIAZEPAN-1-YL)-1H-BENZO[D]IMIDAZOLE, 95%, 25mg](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4856183328.png "1-(2-ETHOXYETHYL)-2-(4-METHYL-1,4-DIAZEPAN-1-YL)-1H-BENZO[D]IMIDAZOLE, 95%, 25mg")
Impulsada por Lightspeed
Mostrar precios en:EUR
