monoclonal de ratón anti-Perilipin 2 (N-terminal), AP125, 0,2 ml
REF 1NP4.1
€ 132,31
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monoclonal de ratón anti-Perilipin 2 (N-terminal), AP125, 0,2 ml
Información del producto
Código hs: 30021500
Marca: PROGEN
anti-Perilipin 2 (N-terminus) mouse, monoclonal, AP125, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125 50 μg/ml, monoclonal, affinity purified
Perilipin 2/Adipophilin/ADRP/PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Enhanced expression of Perilipin 2/Adipophilin/ADRP/PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets.
Polypeptide reacting: Perilipin 2/Adipophilin/ADRP/PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.
Immunolocalization: Perilipin 2/Adipophilin/ADRP/PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages.
Tested cultured cell lines: PLC, MDCK.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: AP125
Immunogen: synthetic peptide (aa 5-27 from N-terminus of human adipophilin/PLIN2)
UniprotID: A0A5F4CRI5 (dog, canis familiaris), Q99541 (human), Q5U2U5 (rat)
Synonym: perilipin-2, adipophilin, adipose differentiation-related protein, ADRP, PLIN2, ADFP
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IHC, WB
Reactivity: dog, human, rat
No reactivity: bovine
Toepassing
Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0,5-5 μg/ml; microwave treatment recommended)
Western Blot (WB): 1:50-1:100 (0.5-1 μg/ml)
Western blot analysis of HepG2 cell lysate with anti-Perilipin 2 antibody. Western blot analysis was performed on 15 μg HepG2 lysate and 15 μg HepG2 + OA lysate. Cells were previously treated with 400 μM oleic acid (OA) if indicated. Cells were lysed in RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125 (Cat. No. 690102) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated at 4°C over-night. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
IHC of rat stomach (courtesy of J. Heß, University Hospital Heidelberg)
Geen medisch hulpmiddel / Geen ivD-product
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