monoclonal de ratón anti-Vimentina, VIM 3B4, 0,2 ml
REF 1NP3.1
€ 110,31
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monoclonal de ratón anti-Vimentina, VIM 3B4, 0,2 ml
Información del producto
Código hs: 30021500
Marca: PROGEN
anti-Vimentin mouse monoclonal,, VIM 3B4, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Vimentin mouse monoclonal, VIM 3B4 50 μg/ml, monoclonal, affinity purified
The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
Tested cultured cell lines: fibroblasts (SV-80).
Bohn W, Wiegers W, Beuttenmüller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992). Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).
Host: mouse
Antibody type: monoclonal
Isotype: IgG2a kappa
Clone: VIM 3B4
Immunoge: vimentin purified from bovine lens
UniprotID: P48616 (bovine), P09654 (chicken), P08670 (human)
Synonym: vimentin, VIM
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: amphibia, bovine, chicken, human, monkey, mouse
Toepassing
Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:100-1:200 (250-500 ng/ml)
Immunohistochemistry (IHC) - paraffin: 1:100-1:200 (250-500 ng/ml, protease treatment and/or microwave treatment recommended)
Western Blot (WB): 1:500-1:5,000 (10-100 ng/ml)
Human skin (courtesy of J. Heß, University Hospital Heidelberg)
Mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)
Western blot analysis of HeLa lysate with anti-Vimentin antibody. Western blot analysis was performed on 10 μg wild type (WT) and 10 μg Vimentin knockout (KO) HeLa lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Vimentin mouse monoclonal, VIM 3B4 (Cat. No. 690013) was diluted in blocking buffer (antibody concentration 33 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
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