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Kit de prolifération de cellules ClickTech EdU T 488, pour 192 tests.

Kit de prolifération de cellules ClickTech EdU T 488, pour 192 tests.

Référence 1Y7X.2
€ 609,64
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Kit de prolifération de cellules ClickTech EdU T 488, pour 192 tests.
Détails du produit
Formules chimiques: MgCl2
Code SH: 38221900
Marque: baseclick
ClickTech EdU T cell Kit 488, for Flow Cytometry, Storage temp. +4 °C, UN-Nr. 3077

Especially for the investigation of cell proliferation in T cells, baseclick offers the three kits below with different fluorescence markers. In general, T-cell proliferation is of high interest for research in immunology. These kits offer numerous advantages over previous approaches, such as low toxicity or the outstanding signal-to-noise ratio.



ClickTech EdU T Cell Proliferation Kit 488 for Flow Cytometry

The kit contains
  • 5-Ethynyl-deoxyuridine (5-EdU) (20x)
  • Eterneon2 GREEN Azide
  • Reactor system
  • Saponin-based reagent (10x solution)
  • Fixative solution (4 % Paraformaldehyde)
  • Buffer additive
  • Reaction buffer (10x)


Technische informatie
λ 488 nm
Markeerstiften Eterneon² GREEN Azide
Click Chemistry

The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.


References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.


Click Chemistry

In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.


Tip:

The number at the end of the product name specifies the excitation wavelength of the included fluorescent marker. Suitable excitation source are:

  • 488 nm (blue-green argon laser 488 nm, blue diode laser 473 nm)
  • 555 nm (green Nd:YAG laser 532 nm, green helium-neon-laser 543 nm)
  • 594 nm (yellow helium-neon-laser 594 nm, yellow diode laser 594 nm)
  • 647 nm (red krypton laser 647 nm, red helium-neon-laser 633 nm, red diode laser 635 or 650 nm)

  • Cell Proliferation Assays

    The ClickTech EdU Cell Proliferation Kits for EdU mediated detection of cell proliferation provide a superior alternative to time-consuming and less sensitive bromodeoxyuridine (BrdU) assays. Similar to BrdU assays, these assays utilise a uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities and to the assay situation (e.g. for double or triple detection). Furthermore, the click reaction is highly selective and therefore prevents any unspecific labelling. The protocol includes only few steps and decreases the total amount of time.


    Kits are available for the following applications:

    ▶ Kits for cell proliferation: • T cell Proliferation (ClickTech EdU T Cell Proliferation Kit - Flow Cytometry) • Fluorescence microscopy (ClickTech EdU Cell Proliferation Kit - Imaging) • High Throughput Screening (ClickTech EdU Cell Proliferation Kit - HTS) • Flow cytometry (ClickTech EdU Cell Proliferation Kit - Flow Cytometry)▶ Kits for nucleic acid modification: • PCR Modification • DNA FISH Kit • RNA Labeling Kit • ClickTech Oligo Link Kit • ClickTech Library Kit - full-length mRNA Seq


    Advantages of the click chemistry based approach:


    Advantages of the click chemistry based method:

    • Highly reliable
    • Easy handling
    • Fast detection procedure (only 30 min.)
    • Mild conditions, no DNA denaturation required
    • Modular system in click chemistry
    • Compatible with various dyes
    • Compatible with multiplexing

    Code in product name Markers Absorption (nm) Emission (nm) Filter configuration
    -488 6-FAM azide 496 516 FITC
    -555 5-TAMRA azide 546 579 Cy3™
    -594 5/6 Sulforhodamine 584 603 Texas Red®
    -647 Eterneon red (analogous to cyanine 5 azide) 643 662 Cy5T™

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