ROTI®Bloc, 250 ml

Protein-free blocking solution for all blot and hybridisation systems. Diluted also perfectly suitable as base solution for immunochemical detections.

In order to obtain high signal intensity, nonspecific antibody binding sites must be blocked. Whilst detergents are not effective as blocking agents, the addition of proteins such as BSA may mask specific binding sites.

ROTI^®Block is an innovative, polymer-based blocking reagent which solves such problems. With ROTI^®Block, the specific binding sites remain accessible while nonspecific reactions are suppressed, thus leading to an increase in signal intensity.

ROTI^®Block is a protein-free blocking solution that can be used diluted as a base solution for all steps of Western detections and ELISAs. ROTI^®Block replaces PBS/TBS buffers and other buffer systems including the blocking reagents used.
If required, ROTI^®Block can be enriched with further blocking reagents, detergents etc. to further optimise the detection system.

Included you will find a detailed instruction manual with protocols, e.g. for stripping ROTI^®PVDF-membranes from bound probes without destabilising the blocking, which enables fast rescreening of the filter.
ROTI^®Block also enables the blot to be Ponceau stained even after blocking.
For detection systems using antibodies specifically directed against protein-phosphorylations we recommend to test and optimise the blocking efficiency prior to assaying your samples.