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Solution ROTI®Cell Trypsine (1x)

Solution ROTI®Cell Trypsine (1x)

Référence 1Y17.1
€ 21,78
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Solution ROTI®Cell Trypsine (1x)
Détails du produit
Code SH: 35079090
Marque: Carl Roth
ROTI®Cell Trypsin solution (1x), sterile, 1x, CELLPURE®, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. -20 °C
Steriel

Trypsin is a protein-cleaving digestive enzyme of the pancreas belonging to the serine proteases, which cleaves lysine and arginine at the C-terminus. Trypsin solutions are used for the separation of cells from tissue or cell monolayers. Thus, they are essential for routine applications in cell culture passaging, for cell culture experiments to obtain an adequate number of cells or for dissociation of primary tissue.
As an alternative to Trypsin, the morecell-protective solution ROTI®Cell Accutase solution can be used, which is free of mammalian components.



ROTI®Cell Trypsin solution (1x) CELLPURE® 0.25 % in DPBS, ready-to-use, sterile

The required trypsin concentration depends primarily on the cell type and the age of the cell culture. For orientation, you will find a complete protocol in the technical info brochure.


After cell dissociation, trypsin should be inhibited. This can be done either by using FCS or trypsin inhibitors.



Technische informatie
Enzyme Trypsin from porcine pancreas
Synonym Cell dissociation reagents
Toepassing Cell dissociation
Gebruikstip Preparation of aliquots recommended to avoid repeated freeze-thaw cycles.
pH value 7.0-7.6
Osmolality 260-340 mOsm/kg
Endotoxins ≤10.0 EU/ml
Sterility complies
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