Name: ROTI®Load DNAstain 3 SYBR® Vert, 9,0 ml, 5 x 1,8 ml
Brand: Carl Roth
SKU: 1CN7.2
Price: 178.31 EUR
Availability: InStock

ROTI®Load DNAstain 3 SYBR® Vert, 9,0 ml, 5 x 1,8 ml

Détails du produit

Code SH: 38229000

Marque: Carl Roth

ROTI®Load DNAstain 3 SYBR Green,, 6x DNA gel loading buffer, ready-to-use, DNase-free, Density (D) ~1,115 g/cm³, Storage temp. -20 °C

Loading bufer with the fluorescent dye SYBR^® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels.

Alternative for ethidium bromide, non-toxic, non-mutagen
20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band
For use with common broad-band ethidium bromide photo filters
Excitation possible via UV light (254 nm) and blue light (495 nm)
Compatible with all usual down-stream applications.

ROTI^®Load DNAstain SYBR^® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR^® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR^® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects.

SYBR^® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE^®PROfessional runVIEW, Art. No. 4849.1).
When excited, nucleic acid-bound SYBR^® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR^® Green foto filters.

The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI^®Load DNAstain SYBR^® Green.

Golflengte

Excitation maximum (bound to DNA): 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm

ROTI^®Load DNAstain 3 SYBR^® Green 6x conc., ready-to-use

Gebruiksinstructies

ROTI^®Load DNAstain 3 SYBR^® Green is a special gel loading buffer with orange G as the only tracking dye optimized for very short runs. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs. Also well suited if bromophenol blue or xylene cyanol could mask important DNA bands.

Glycerin is the standard reagent for increasing sample density.

Recommended for staining of dsDNA- and dsRNA-fragments of under 500 bp.

Technische informatie

Activeringsmax. (DNA-gebonden)	~320 nm 470-500 nm
Aantal sporen	ca. 1000/ml
Emissiemaximum	525 nm
Kleurstof	Orange G, SYBR^® Green
Incl. DNA-kleuring	ja
Sensitiviteit	0,01 ng/band
Toepassing	Simultaneous gel staining, small fragments

Selecting a Suitable ROTI^®Load Gel Loading Buffer for Nucleic Acids

Care should be taken to ensure that the dyes contained in the gel stop before smallest relevant DNA bands. This ensures that the buffer can be stopped in time. However, the selected dyes may overlay the bands shown. In this case, select a loading buffer that does not contain any unwanted dyes.
Bromophenol blue and xylene cyanol can be used as colour markers in all standard gels. If a relevant band is overlaid by one of these colour markers, choose a gel loading buffer containing Orange G. If small fragments are to be assayed, the gel loading buffer should contain Orange G in order to mark the run. If relevant bands are overlaid, however, a choice can be made between bromophenol blue or xylene cyanol depending on the size of the bands. A loading buffer without xylene cyanol is usually used for assaying large fragments.

Glycerine is the standard reagent for increasing the density of samples.
Ficoll^® 400 produces particularly well-defined bands.

Summary Staining of Nucleic Acid in Gels

Product	Sensitivity	Description	Excitation
Ethidium bromide solution	0.5-5 ng/band	Standard fluorescent staining	254-330 nm, 500 nm UV- and blue light
SYBR^® Green DNA dye	0.01 ng/band	Non-toxic, green fluorescent staining	254 nm and 495 nm UV- and blue light
ROTI^®GelStain	0.3 ng/band	Non-toxic, green fluorescent staining	302 nm and 490 nm UV- and blue light
ROTI^®GelStain Red	0.3 ng/band	Non-toxic, red fluorescent staining	310 nm, 540 nm UV- and blue light
ROTI^®Methylene blue staining concentrate	10 ng/band	Reversible, non-toxic blue staining	White light
ROTI^®Black N	<0.1 ng/band	Silver staining of DNA polyacrylic amide gels	White light

Appearance	orange solution
Each batch is functionally tested for its suitability in electrophoresis.