ROTI®Concentré de coloration au bleu de méthylène, 12 ml
Référence 0648.2
€ 155,31
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ROTI®Concentré de coloration au bleu de méthylène, 12 ml
Détails du produit
Phrases P: P202 P280 P303+P361+P353 P304+P340 P305+P351+P338 P308+P313
Phrases H: H302-H314-H341-H373-H412 EUH071
Classe de danger: 8 III
Code SH: 29054500
Marque: Carl Roth
ROTI®Methylene blue, staining concentrate, 200x conc., Density (D) ~0,97 to 1 g/cm³, Flash point (flp) 40 °C, Boiling point (bp) 100 °C, Storage temp. +4 °C, UN-Nr. 1170
ROTI®Methylene blue staining concentrate 200x conc.
ROTI®Methylene blue staining concentrate was specially developed for sensitive staining of DNA in agarose gels. Sensitivity reaches up to 10 ng per band. Staining can be conducted in a simple staining bowl, a transilluminator is not required.
- Highly reversible alternative to ethidium bromide
- High sensivity (in minimum 10 ng/band)
- No transilluminator required
- Solution can be used repeatedly
- Sufficient for more than 50 mini gels
Mechanisme
ROTI®Methylene blue staining concentrate binds to the negativly charged DNA backbone by salt bridges formed through quaternary ammonia complexes. The staining is very easy to remove by washing or replacement by salts.
Gebruiksinstructies
For staining DNA agarose gels, DNA polyacrylamide gels, for reversible staining of Southern- and Northern-Blots.
Staining can be performed in a simple staining dish, a transilluminator is not required. The diluted staining solution can be used several times.
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
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