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ROTI®Concentré de coloration au bleu de méthylène, 3 ml

ROTI®Concentré de coloration au bleu de méthylène, 3 ml

Référence 0648.1
€ 54,68
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ROTI®Concentré de coloration au bleu de méthylène, 3 ml
Détails du produit
Code SH: 29054500
Marque: Carl Roth
ROTI®Methylene blue, staining concentrate, 200x conc., Density (D) ~0,97 to 1 g/cm³, Flash point (flp) 40 °C, Boiling point (bp) 100 °C, Storage temp. +4 °C, UN-Nr. 1170


ROTI®Methylene blue staining concentrate 200x conc.

ROTI®Methylene blue staining concentrate was specially developed for sensitive staining of DNA in agarose gels. Sensitivity reaches up to 10 ng per band. Staining can be conducted in a simple staining bowl, a transilluminator is not required.

  • Highly reversible alternative to ethidium bromide
  • High sensivity (in minimum 10 ng/band)
  • No transilluminator required
  • Solution can be used repeatedly
  • Sufficient for more than 50 mini gels

Mechanisme

ROTI®Methylene blue staining concentrate binds to the negativly charged DNA backbone by salt bridges formed through quaternary ammonia complexes. The staining is very easy to remove by washing or replacement by salts.


Gebruiksinstructies

For staining DNA agarose gels, DNA polyacrylamide gels, for reversible staining of Southern- and Northern-Blots.
Staining can be performed in a simple staining dish, a transilluminator is not required. The diluted staining solution can be used several times.



Summary Staining of Nucleic Acid in Gels

Product Sensitivity Description Excitation
Ethidium bromide solution 0.5-5 ng/band Standard fluorescent staining 254-330 nm, 500 nm UV- and blue light
SYBR® Green DNA dye 0.01 ng/band Non-toxic, green fluorescent staining 254 nm and 495 nm UV- and blue light
ROTI®GelStain 0.3 ng/band Non-toxic, green fluorescent staining 302 nm and 490 nm UV- and blue light
ROTI®GelStain Red 0.3 ng/band Non-toxic, red fluorescent staining 310 nm, 540 nm UV- and blue light
ROTI®Methylene blue staining concentrate 10 ng/band Reversible, non-toxic blue staining White light
ROTI®Black N <0.1 ng/band Silver staining of DNA polyacrylic amide gels White light

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