ROTI®Pol Hot-TaqS Red-Mix (2x), 10 ml, 10 x 1 ml

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI^®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI^®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

Optimized pre-mixed 2x PCR solution containing the recombinant Hot-TaqS DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl₂, and all other components required for PCR (w/o primers and template DNA), plus components for direct gel electrophoresis. ROTI^®Pol Hot-TaqS Red-Mix (2x) is recommended for use in highly specific PCR applications immediately followed by gel electrophoresis.

PCR assaying with ROTI^®Pol Hot-TaqS Red-Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI^®Pol Hot-TaqS Red-Mix (2x) is superior for use in all standard Taq-based cycling protocols when big sample numbers shall be amplified with high specificity as well as high reproducibility. ROTI^®Pol Hot-TaqS Mix (2x) master mix is, therefore, the optimal choice for high throughput PCR assays in colony screening, prior to sequencing, in cycle sequencing, and in similar assays.

Due to the optimized composition of the master mix combined with the antibody-blocked Hot-TaqS polymerase, ROTI^®Pol Hot-TaqS Red-Mix (2x) delivers highly specific PCR amplification of good yield with a wide range of PCR templates. The antibody-mediated blocking of the DNA polymerase is released only at the initial denaturation step, hence resulting in highly specific amplification of the target sequence without production of unwanted side products caused by unspecific primer annealing.

ROTI^®Pol Hot-TaqS Red-Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA and up to 5 kb with Lambda DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The Hot-TaqS polymerase included in the master mix possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

Compositon:
2 or 10 vials, respectively, with 1,25 ml each of ROTI^®Pol Hot-TaqS Red-Mix (2x) containing Hot-TaqS polymerase, 0,4 mM each dNTP, and 4 mM MgCl₂ in 2x reaction buffer with 0,02 % cresol red.