Rhodamine-12-dUTP, 50 µl, 50 nmoles

All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also “long range PCR”, repeated quantitative light-cycling reactions, and tests for physical stability.

Rhodamine-12-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. Detection of labelled nucleic acids can easily be done by direct analysis of fluorescent signals (e. g. by fluorescence microscopy).
Rhodamine-12-dUTP can also be used for double staining techniques in combination with fluorescein-12-dUTP or biotin-11-dUTP and corresponding antibodies or streptavidin-complexes, respectively.

Excitation: 505 nm
Emission: 530 nm (red)

Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment).
Tested for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase.

Rhodamine Green, mixture of 5/6 isomeres

ε₅₀₅ (pH 7) = 8,5 E x mmol^-1 x cm^-1; pH: 7,5 ±0,2