Name: Albumine sérique bovine (BSA) Fraction V, sulfhydryle bloqué, origine Nouvelle-Zélande, 200 g
Brand: Carl Roth
SKU: 1ET3.4
Price: 772.48 EUR
Availability: InStock

Albumine sérique bovine (BSA) Fraction V, sulfhydryle bloqué, origine Nouvelle-Zélande, 200 g

Détails du produit

Numero CAS: 9002-18-0

Code SH: 35029020

Marque: Carl Roth

Bovine Serum Albumin (BSA) Fraction V,, sulfhydryl blocked, NZ-Origin, min. 98 %,, for biochemistry and molecular biology, Molar mass (M) ~66 000 g/mol, Storage temp. +4 °C, CAS No. [9048-46-8], EG-Nr. 232-936-2

For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.

Molar extinction coefficient: 4,4x10⁴ L x mol^-1 x cm^-1 (acc. to 0,667 L x mol^-1 x cm^-1 for a 0,1 % solution).

Bovine Serum Albumin (BSA) Fraction V, sulfhydryl blocked, NZ-Origin ≥98 %, for biochemistry and molecular biology

High quality albumin originating from Australia or New Zealand.
By blocking the thiol groups, the polymerisation of the albumin molecules is very efficiently prevented, which can occur during the storage of powder and solution and change the properties of the albumin. The albumin remains stable over time as a monomer and can, therefore, be used very well for reproducible laboratory assays. Due to its low IgG and protease content, the albumin is excellent for all immunoassays such as WESTERN or ELISA and as a stabilising reagent for proteins, antibodies etc. Furthermore, the Albumin, sulfhydryl-blocked, NZ-Origin is recommended for all thiol or sulfur sensitive assays.

Tested for the absence of detectable free sulfhydryl groups.

Recommended Application of Bovine Serum Albumins (BSA)

BSA	Art. No.	Recommended Application
Albumin Solution 20 %, Europe	9400	Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply.
Albumin Solution 30 %, Europe	9401	Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply.
Albumin Solution 30 %, with azide, US-Origin	9414	Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply. Recommended if albumin with certified origin USA has to be used.
Fraction V, Europe	1ETA	Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply.
Fraction V, US-Origin	3854	Stabilisation of proteins and blocking reagent in all standard biochemical detection systems. Basically suitable for all assays unless specific requirements apply. Recommended if albumin with certified origin USA has to be used.
Fraction V, NZ-Origin	8076	Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, pH 5.2, Europe	2834	Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs.
Fraction V, pH 5.2, US-Origin	1ET8	Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs. Recommended if albumin with certified origin USA has to be used.
Fraction V, sufhydryl-blocked, NZ-Origin	1ET3	Particularly sensitive assays where monomeric albumin is guaranteed to be used or where reproducibility is particularly important. Can be stored as monomers over a longer period of time.
Fraction V, biotin-free, NZ-Origin	0163	Stabilisation of proteins and blocking reagent in all biotin / streptavidin-mediated detection systems (membrane-bound- or coating-plate-assays, as well as soluble assays).
Fraction V, protease-free, Europe	T844	Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays.
Fraction V, protease-free, US-Origin	1ET5	Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Recommended if albumin with certified origin USA has to be used.
Fraction V, protease-free, NZ-Origin	1ET7	Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, IgG-free, US-Origin	1ET9	Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals. Recommended if albumin with certified origin USA has to be used.
Fraction V, IgG-free, NZ-Origin	3737	Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, fatty acid-free, Europe	0052	Stabilisation of proteins and blocking reagent in detection systems. Particularly recommended for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins.
Fraction V, fatty acid-free, US-Origin	9638	Particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Also well suited for cell biology and all antibody-mediated detection systems. Recommended if albumin with certified origin USA has to be used.
Fraction V, fatty acid-free, NZ-Origin	1ETC	Stabilisation of proteins and blocking reagent in detection systems. Particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, very low endotoxin, US-Origin	1ET6	Cell culture of all cell lines, primary cells, stem cells. Cloning and 3D-cultures or cell-stressing assays like transfections, in vitro infections, cell fusion. Recommended if albumin with certified origin USA has to be used.
Fraction V, very low endotoxin, NZ-Origin	1ET4	Cell culture of all cell lines, primary cells, stem cells. Cloning and 3D-cultures or cell-stressing assays like transfections, in vitro infections, cell fusion. Recommended if albumin with certified origin New Zealand or Australia has to be used..

Molar extinction coefficient: 4,4x10⁴ L x mol^-1 x cm^-1 (acc. to 0,667 L x mol^-1 x cm^-1 for a 0,1 % solution).

BSA are proteins yielded from blood, which function as binding and transport proteins in blood circulation. Depending on the preparation process, the albumins contain different quality and quantities of metabolic products, enzymes, peptides, fatty acids, vitamines, etc. and are suitable for different applications.

Heatshock albumin

For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.

Albumin, NZ-Origin is particularly recommended when procedural regulations require the use of albumin of guaranteed origin Australia or New Zealand.

BSA, US-Origin is particularly recommended if the use of albumin with certified US-Origin is dictated by process regulations.

Albumin of European origin is produced from blood of European cattle herds. It is excellent for use in all assays and products manufactured for regions outside the USA, Australia and New Zealand.

Appearance	powder to flakes
Colour	pale yellow to greenish
Origin	Australia/New Zealand
Assay	≥98 %
Protein	≥98 %
Loss on drying	≤5 %
pH-Value (7 % in H2O)	6.5-7.5
Sodium (Na)	≤15 mg/g
Chloride (Cl)	≤6 mg/g
IgG (COBAS)	none detected
Protease	none detected
Free sulfhydryl	≤0.1 mol/mol
Extinction (DTNB assay)	≤1.0