Uréase
Référence 6065.1
€ 569,40
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Uréase
Détails du produit
Code SH: 35079090
Marque: Carl Roth
Urease, min. 220 Nessler U/mg material, 0,25 MU, Molar mass (M) ~480000 g/mol, Storage temp. -20 °C, CAS No. [9002-13-5], EG-Nr. 232-656-0
Hydrolase belonging to the Amidases. Metallo-enzyme. Urease hydrolasis urea, resulting in ammonia and carbon dioxide, or in ammonia- and carbonate ions, respectively. The molecule isolated from Jack beans is composed of 6 subunits of approx. 91 kDa each with an overall size of approx. 544 kDa. Nickel is the cofactor.
Using high amounts of urea as substrate (200 mM), urease is applied for visualisation of the connection between reaction rate and time. Detection is carried out via measurement of conductivity, or, photometrically, via colour change of bromothymol blue. Furthermore, model system for catalytic activation of carbon dioxide in metallo-organic chemistry.
Gebruiksinstructies
pH optimum: 7.4.
Recommended reaction buffer system is 40 mM potassium phosphate buffer, pH 7.4. Heavy metals (e. g. lead) are inhibitory.
Urease ≥220 Nessler U/mg material
Unit-definitie
One unit (U) is defined as the amount of enzyme that catalyzes the liberation of 1 µmole of NH3 per min at pH 7.0 and 25 °C.
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
| Appearance | white material |
| Activity | ≥220 Nessler U/mg substance |
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