monoclonal de souris anti-Cardiac Actin, AC1-20.4.2, 0,2 ml
Référence 1NLN.1
€ 110,31
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monoclonal de souris anti-Cardiac Actin, AC1-20.4.2, 0,2 ml
Détails du produit
Code SH: 30021500
Marque: PROGEN
anti-Cardiac Actin mouse, monoclonal, AC1-20.4.2, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 50 μg/ml, monoclonal, affinity purified
Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied.
Polypeptide reacting: specific for fetal (cardiac) isoform of actin.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: AC1-20.4.2
Immunogen: synthetic NH2 terminus decapeptide of cardiac isoform of actin
UniprotID: P68034 (chicken), P68032 (human), G1STB6 (rabbit)
Synonym: actin, alpha cardiac muscle 1, alpha-cardiac actin [cleaved into: actin, alpha cardiac muscle 1, intermediate form], ACTC1, ACTC
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IHC, WB
Reactivity: bovine, chicken, human, mouse, rabbit
Toepassing
Immunohistochemistry (IHC) - frozen: 1:10 (5 μg/ml; include 0.5 M NaCl in all washing buffers to enhance specificity)
Immunohistochemistry (IHC) - paraffin: 1:10 (5 μg/ml; microwave treatment recommended)
Western Blot (WB): 1:1,000 (50 ng/ml; include 1 M NaCl in all washing buffers to enhance specificity)
Western blot analysis of human heart whole tissue lysate and with anti-Cardiac Actin antibody. Western blot analysis was performed on 20 μg human heart lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 (Cat. No. 690075) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, liquid, purified
Geen medisch hulpmiddel / Geen ivD-product
| Clonality | Monoclonal |
| Concentration | 50 µg/ml |
| Raised in | Hybridoma cell culture supernatant |
| Immunglobulin (Ig) class | IgG1 |
| Clone | AC1-20.4.2 |
| Conjugate | Unconjugated |
| Purification | Affinity chromatography |
| Purity (SDS-PAGE) | ≥95 % |
| PBS buffer, pH 7.4 with 0.09 % sodium azide and 0.5 % BSA., Short term at 2-8 °C (+4 °C); long term storage in aliquots at -20 °C; avoid freeze/thaw cycles. | |
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