anti-Cyclin-Dependent Kinase 4 monoclonal de souris, DCS-156, 1 ml
Référence 1NNE.2
€ 228,93
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anti-Cyclin-Dependent Kinase 4 monoclonal de souris, DCS-156, 1 ml
Détails du produit
Code SH: 30021500
Marque: PROGEN
anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Cyclin-Dependent Kinase 4 mouse monoclonal, DCS-156 50 μg/ml, monoclonal, affinity purified
DCS-156 reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. In immunohistochemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) and cervix DCS-156 shows distinct nuclear staining, especially in tumor areas of enhanced proliferation.
Epitope recognized: aa 270-290.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: DCS-156
Immunogen: human recombinant full-length cdk4 polypeptide
UniprotID: P11802 (human),P30285 (mouse),P35426 (rat)
Synonym: cyclin-dependent kinase 4, EC 2.7.11.22, cell division protein kinase 4, PSK-J3,
DK4
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: WB
Reactivity: human, mouse, rat
Toepassing
Western Blot (WB): 1:50-1:500 (0.1-1 μg/ml)
Western blot analysis of human HeLa cell lysate with anti-Cyclin-Dependent Kinase 4 antibody. Western blot analysis was performed on either 5 μg or 10 μg of HeLa lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Cyclin-Dependent Kinase 4 mouse monoclonal, DCS-156 (Cat. No. 690096) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
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