monoclonal de souris anti-6-His-tag, 6His, 100 µg
Référence 1NNA.2
€ 454,37
En stock
1
Enregistrer ce produit pour plus tard
monoclonal de souris anti-6-His-tag, 6His, 100 µg
Détails du produit
Phrases P: P260 P270 P280 P302+P352 P305+P351+P338 P310
Phrases H: H302+H312+H332-H314-H412 EUH032
Numero CAS: 593-84-0
Formules chimiques: C2H6N4S
Classe de danger: 8 II
Code SH: 30021500
Marque: PROGEN
anti-6-His-tag mouse, monoclonal, 6His, after dissolv. in 400 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-6-His-tag mouse monoclonal, 6His lyophilized, monoclonal, affinity purified
The monoclonal 6-His antibody recognizes polyhistidine (6-His). The 6-His tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: 6His
Immunogen: recombinant protein containing the sequence HHHHHH
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IP, WB
Reactivity: 6-His
Toepassing
Immunocytochemistry (ICC): assay dependent
Immunoprecipitation (IP): assay dependent
Western Blot (WB): 1:2,000-1:5,000 (0.125-0.05 μg/ml)
Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 160 ng, 80 ng or 20 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.125 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 50 ng or 25 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
Vous aimerez peut-être aussi
(3,5-Dichloro-4-methylphenyl)methanol, 98%, 250mg
(3,5-Dichloro-4-methylphenyl)methanol, 98%, 250mg
Référence F761489-250MG
€ 1 246,30
3-(3-Fluoro-4-methylphenyl)piperidine, 97%, 250mg
3-(3-Fluoro-4-methylphenyl)piperidine, 97%, 250mg
Référence F688377-250MG
€ 513,70
Ion exchange resin ROTI®Change MPC, 5 kg
Ion exchange resin ROTI®Change MPC, 5 kg
Référence 1YE1.2
€ 626,91
![6-[4-(2-Methoxyethoxy)phenyl]-6-oxohexanoic acid, 97.0%, 1g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4868314057.png "6-[4-(2-Methoxyethoxy)phenyl]-6-oxohexanoic acid, 97.0%, 1g")
6-[4-(2-Methoxyethoxy)phenyl]-6-oxohexanoic acid, 97.0%, 1g
6-[4-(2-Methoxyethoxy)phenyl]-6-oxohexanoic acid, 97.0%, 1g
Référence F207638-1G
€ 1 179,20
1-(dibromomethyl)-4-nitrobenzene, 25g
1-(dibromomethyl)-4-nitrobenzene, 25g
Référence F373492-25G
€ 1 710,50
5-(Difluoromethyl)-1H-pyrazol-3-amine, 95.0%, 250mg
5-(Difluoromethyl)-1H-pyrazol-3-amine, 95.0%, 250mg
Référence F332020-250MG
€ 231,00
![1-tert-butyl-4-isopropyl-1,6-dihydro-7H-pyrazolo[3,4-d]pyridazin-7-one, 95.0%, 250mg](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4856823357.png "1-tert-butyl-4-isopropyl-1,6-dihydro-7H-pyrazolo[3,4-d]pyridazin-7-one, 95.0%, 250mg")
![{[4-(2-methylpropyl)phenyl]methyl}(prop-2-en-1-yl)amine, 95.0%, 1g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4856235602.png "{[4-(2-methylpropyl)phenyl]methyl}(prop-2-en-1-yl)amine, 95.0%, 1g")
![{4-[(Methylsulfonyl)oxy]phenyl}acetic acid, 97%, 10g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4856229342.png "{4-[(Methylsulfonyl)oxy]phenyl}acetic acid, 97%, 10g")
{4-[(Methylsulfonyl)oxy]phenyl}acetic acid, 97%, 10g
{4-[(Methylsulfonyl)oxy]phenyl}acetic acid, 97%, 10g
Référence F609907-10G
€ 1 233,10
Propulsé par Lightspeed
Afficher les prix en :EUR
