monoclonal de souris anti-DDDDK-tag, AP1501, 100 µg
Référence 1NLE.2
€ 454,37
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monoclonal de souris anti-DDDDK-tag, AP1501, 100 µg
Détails du produit
Numero CAS: 147-85-3
Formules chimiques: C5H9NO2
Code SH: 30021500
Marque: PROGEN
anti-DDDDK-tag mouse, monoclonal, AP1501, after dissolv. in 400 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-DDDDK-tag mouse monoclonal, AP1501 lyophilized, monoclonal, affinity purified
The monoclonal AP1501 antibody recognizes the amino acid sequence DDDDK (Flag). The DDDDK-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG2b
Clone: AP1501
Immunogen: recombinant protein containing the sequence DDDDK
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IP, WB
Reactivity: DDDDK
Toepassing
Immunocytochemistry (ICC): assay dependent
Immunoprecipitation (IP): assay dependent
Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)
Western blot analysis of E. coli lysate containing DDDDK-tagged protein with anti-DDDDK-tag antibody. Western blot analysis was performed on 80 ng, 40 ng or 10 ng of E. coli lysate containing DDDDK-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5 % dry milk in PBST for 1 h at RT. The primary antibody anti-DDDDK-tag mouse monoclonal, AP1501 (Cat. No. 910DDKL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
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