anti-HA-tag souris monoclonal, 12CA5, 100 µg
Référence 1NN7.2
€ 412,97
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anti-HA-tag souris monoclonal, 12CA5, 100 µg
Détails du produit
Phrases P: P280 P302+P352 P304+P340 P305+P351+P338
Phrases H: H315-H319-H335
Numero CAS: 14933-09-06
Formules chimiques: C19H41NO3S
Code SH: 30021500
Marque: PROGEN
anti-HA-tag mouse, monoclonal, 12CA5, after dissolv. in 400 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-HA-tag mouse monoclonal, 12CA5 lyophilized, monoclonal, affinity purified
The monoclonal 12CA5 antibody recognizes the HA-tag (hemagglutinin tag). The HA-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG2b
Clone: 12CA5
Immunogen: 9 amino acids from the human influenza virus hemagglutinin protein
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: WB
Reactivity: HA
Toepassing
Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)
Western blot analysis of E. coli lysate containing HA-tagged protein with anti-HA-tag antibody. Western blot analysis was performed on 10 ng or 5 ng of E. coli lysate containing HA-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5 % dry milk in PBST for 1 h at RT. The primary antibody anti-HA-tag mouse monoclonal, 12CA5 (Cat. No. 910HAL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
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