Homepage/Negozio/Scienza di vita/Biochimico/Western ed Elisa
3,3,5,5-Tetrametilbenzidina, 1 g, vetro

3,3,5,5-Tetrametilbenzidina, 1 g, vetro

Codice articolo 6350.1
€ 69,60
Disponibile
1
Salva questo articolo per dopo
3,3,5,5-Tetrametilbenzidina, 1 g, vetro
Dettagli del prodotto
Numero CAS: 9048-46-8
Codice SA: 29215990
Marca: Carl Roth
3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., Molar mass (M) 240,35 g/mol, Storage temp. +4 °C, CAS No. [54827-17-7], EG-Nr. 259-364-6, Empirical formula C16H20N2


3,3',5,5'-Tetramethylbenzidine ≥98 %, p.a.

Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine.


Dissolves easily in acetone, chloroform and ethanol.


Gebruiksinstructies

Working solution: 1 mg TMB in 0.1 ml dimethyl sulfoxide, add 9.9 ml 0.1 M sodium acetate (pH 6). Filter and add H2O2 ad 0.01 %. Always prepare freshly!
Application: approx. 50 µl per 96well, incubate for 10-30 mins. at room temperature. Add 50 µl 1 M H2SO4 and quantitate photometrically at 450 nm (acc. to: Bos et. al. (1981) J. Immunoassay 2:187).
Protect from light.



Technische informatie
Enzyme Horseradish Peroxidase
Bewijs Colour (blue)
Signaalproduct soluble (ELISA)
Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.


Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos E.S. et al. (1981) J. Immunoassay 2, (3/4), 187.


Appearance beige cryst. powder
Assay (GC) ≥99.0 %
Sulphated ash ≤0,2 %
Loss on drying (3 h, 60 °C, vacuum) ≤1,0 %
Melting range 166-171 °C
Can replace carcinogenic benzidine.
Visualizza altro
Potrebbe anche interessarti
6-Ethoxypyridine-2-carbaldehyde, 95+%, 10g
6-Ethoxypyridine-2-carbaldehyde, 95+%, 10g
6-Ethoxypyridine-2-carbaldehyde, 95+%, 10g
Codice articolo F624837-10G
€ 4 140,40
4-Furan-2-yl-benzonitrile, 250mg
4-Furan-2-yl-benzonitrile, 250mg
4-Furan-2-yl-benzonitrile, 250mg
Codice articolo F031567-250MG
€ 92,40
5-Methyl-1H-pyrazole-4-sulfonyl chloride, 5g
5-Methyl-1H-pyrazole-4-sulfonyl chloride, 5g
5-Methyl-1H-pyrazole-4-sulfonyl chloride, 5g
Codice articolo F890565-5G
€ 1 190,20
(S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-3-(3-carbamoylphenyl)propanoic acid, 97%, 1g
(S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-3-(3-carbamoylphenyl)propanoic acid, 97%, 1g
(S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-3-(3-carbamoylphenyl)propanoic acid, 97%, 1g
Codice articolo F869576-1G
€ 92,40
Thermoshaker Modello Pro – riscaldamento, raffreddamento e agitazione, 1 pezzo
Thermoshaker Modello Pro – riscaldamento, raffreddamento e agitazione, 1 pezzo
Thermoshaker Modello Pro – riscaldamento, raffreddamento e agitazione, 1 pezzo
Codice articolo CPL8.1
€ 2 277,50
6-Bromoindoline, 98.0%, 25g
6-Bromoindoline, 98.0%, 25g
6-Bromoindoline, 98.0%, 25g
Codice articolo F092491-25G
€ 341,00
Numero di colore standard Pt/Co, ROTI®Calipure 40 Hazen, 100 ml, confezione in plastica.
Numero di colore standard Pt/Co, ROTI®Calipure 40 Hazen, 100 ml, confezione in plastica.
Numero di colore standard Pt/Co, ROTI®Calipure 40 Hazen, 100 ml, confezione in plastica.
Codice articolo 1EEE.1
€ 155,31
Sodium Bis(nonafluorobutanesulfonyl)imide>97.0%(T)5g
Sodium Bis(nonafluorobutanesulfonyl)imide>97.0%(T)5g
Sodium Bis(nonafluorobutanesulfonyl)imide>97.0%(T)5g
Codice articolo O0492-5G
€ 224,40
Nicotinic acid hydroxysuccinimidyl ester, 5g
Nicotinic acid hydroxysuccinimidyl ester, 5g
Nicotinic acid hydroxysuccinimidyl ester, 5g
Codice articolo F493404-5G
€ 222,20
1-Tetradecanol>98.0%(GC)25mL
1-Tetradecanol>98.0%(GC)25mL
1-Tetradecanol>98.0%(GC)25mL
Codice articolo T0084-25ML
€ 17,60
N-(2-amino-4-methylphenyl)cyclohexanecarboxamide, 95.0%, 5g
N-(2-amino-4-methylphenyl)cyclohexanecarboxamide, 95.0%, 5g
N-(2-amino-4-methylphenyl)cyclohexanecarboxamide, 95.0%, 5g
Codice articolo F315400-5G
€ 533,50
1-BROMO-3-(DIFLUOROMETHOXY)-5-NITROBENZENE, 95.0%, 250mg
1-BROMO-3-(DIFLUOROMETHOXY)-5-NITROBENZENE, 95.0%, 250mg
1-BROMO-3-(DIFLUOROMETHOXY)-5-NITROBENZENE, 95.0%, 250mg
Codice articolo F303669-250MG
€ 72,60
  • Cerca prodotti
  • Il mio profilo
  • Verifica ordini
  • Preferiti
  • Carrello
Mostra prezzi in:EUR
Passa al contenuto principale
SciSupplies
Negozio
Chi siamo
Contattaci
FAQ
IT
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
Il mio profiloCerca
Carrello
Menu
Parte di HD Beverwijk Trading
Lingua:
IT
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
Termini e condizioniInformativa sulla privacyInformazioni di spedizione e pagamentoPolitica di resoSegnala abuso
Realizzato con Ecwid di Lightspeed