Soluzione HCl-etanolo, 500 ml

After staining tissues are rinsed with HCl-ethanol solution to remove excessive stain. This decolourisation is selective for certain tissue structures retain the stain better than others (differentiation). HCl-ethanol solution is used for regressive hemalum staining, but also for detection of mycobacteria and other stains.

Solution of 3 % hydrochloric acid in ethanol 70 %.