Proteinasi K, 1 g
Codice articolo 3726.2
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Proteinasi K, 1 g
Dettagli del prodotto
Frasi P: P261 P280 P305+P351+P338
Frasi H: H319-H335
Numero CAS: 77-92-9
Formula chimica: C6H8O7
Codice SA: 35079090
Marca: Carl Roth
Proteinase K, lyophil., min. 35 U/mg, BioScience-Grade, Storage temp. -20 °C, CAS No. [39450-01-6], EG-Nr. 254-457-8
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Gebruiksinstructies
Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Proteinase K ≥35 U/mg, BioScience Grade, lyophilised
Endopeptidase for the degradation of proteins with very high solubility, specific activity and DNA purity. Extra high purified.
Voordelen:
- High solubility
- High specific activity
- Extremely low DNA content
Activity: ≥ 35 U/mg lyophilizate
Specific activity: ≥ 45 U/mg protein
DNA content: ≤ 0.1 pg/mg
Gebruiksinstructies
Working solution: 50 µg/ml
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
| Appearance | white powder |
| Activity | ≥35 U/mg |
| Specific activity | ≥45 U/mg |
| Solubility in H2O | ≥50 mg/ml |
| Assay DNA | ≤0.1 pg/mg |
| Exonuclease activity | not detectable |
| Endonuclease activity | not detectable |
| Ribonucleases activity | not detectable |
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