Proteinasi K, 500 mg, 1 x 500 mg
Codice articolo 7528.2
€ 435,98
Disponibile
1
Salva questo articolo per dopo
Proteinasi K, 500 mg, 1 x 500 mg
Dettagli del prodotto
Numero CAS: 0100-10-7
Formula chimica: C9H11NO
Codice SA: 35079090
Marca: Carl Roth
Proteinase K, lyophil., min. 30 mAnson U/mg, Storage temp. +4 °C, CAS No. [39450-01-6], EG-Nr. 254-457-8
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Gebruiksinstructies
Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Proteinase K ≥30 mAnson U/mg, lyophilized
A non-specific endopeptidase with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.
Gebruiksinstructies
Working solution: 50 µg/ml
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
| Activity (Hemoglobin, 37 °C) | ≥30 U/mg |
| DNases, RNases | not detected |
| Endonucleases | not detected |
Potrebbe anche interessarti
3-Chloro-6-(difluoromethoxy)pyridazine, 95%, 500mg
3-Chloro-6-(difluoromethoxy)pyridazine, 95%, 500mg
Codice articolo F618561-500MG
€ 741,40
3-Ethoxyphenyl boronic acid, 98.0%, 25g
3-Ethoxyphenyl boronic acid, 98.0%, 25g
Codice articolo F040251-25G
€ 84,70
![[3-Methoxy-4-(oxan-4-ylmethoxy)phenyl]methanamine hydrochloride, 1g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4856242679.png "[3-Methoxy-4-(oxan-4-ylmethoxy)phenyl]methanamine hydrochloride, 1g")
[3-Methoxy-4-(oxan-4-ylmethoxy)phenyl]methanamine hydrochloride, 1g
[3-Methoxy-4-(oxan-4-ylmethoxy)phenyl]methanamine hydrochloride, 1g
Codice articolo F097096-1G
€ 1 161,60
6-Bromo-3-fluoroisoquinoline, 95.0%, 100mg
6-Bromo-3-fluoroisoquinoline, 95.0%, 100mg
Codice articolo F231196-100MG
€ 37,40
2-Bromo-2'-(trifluoromethyl)acetophenone>98.0%(GC)1g
2-Bromo-2'-(trifluoromethyl)acetophenone>98.0%(GC)1g
Codice articolo B4797-1G
€ 71,50
![1-BOC-6-OXO-1-AZASPIRO[3.3]HEPTANE, 95.0%, 5g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4856939104.png "1-BOC-6-OXO-1-AZASPIRO[3.3]HEPTANE, 95.0%, 5g")
1-BOC-6-OXO-1-AZASPIRO[3.3]HEPTANE, 95.0%, 5g
1-BOC-6-OXO-1-AZASPIRO[3.3]HEPTANE, 95.0%, 5g
Codice articolo F468003-5G
€ 3 777,40
(2R,3S)-3-Benzamido-2-hydroxy-3-phenylpropanoic acid, 95.0%, 500mg
(2R,3S)-3-Benzamido-2-hydroxy-3-phenylpropanoic acid, 95.0%, 500mg
Codice articolo F212819-500MG
€ 64,90
3-Formyl-1H-indole-4-carbonitrile, 97%, 250mg
3-Formyl-1H-indole-4-carbonitrile, 97%, 250mg
Codice articolo F612753-250MG
€ 49,50
End user declaration required
Acido nitrico 25%, puro, 5 l, confezione plastica.
Acido nitrico 25%, puro, 5 l, confezione plastica.
Solo per aziende registrate
Codice articolo 4339.2
€ 62,70
![Glycine, N-[(3?,5?,7?,12?)-3,7,12-trihydroxy-24-oxocholan-24-yl]-, monosodium salt, monohydrate, 97%, 10g](https://d2j6dbq0eux0bg.cloudfront.net/images/88473019/4771232289.png "Glycine, N-[(3?,5?,7?,12?)-3,7,12-trihydroxy-24-oxocholan-24-yl]-, monosodium salt, monohydrate, 97%, 10g")
7-Chloro-3-methyl-1h-indole-2-carboxylic acid, 97%, 250mg
7-Chloro-3-methyl-1h-indole-2-carboxylic acid, 97%, 250mg
Codice articolo F639879-250MG
€ 165,00
Tecnologia Lightspeed
Mostra prezzi in:EUR
