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ROTI®Blue rapido, 1 l, plastica

ROTI®Blue rapido, 1 l, plastica

Codice articolo 4829.2
€ 408,36
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ROTI®Blue rapido, 1 l, plastica
Dettagli del prodotto
Numero CAS: 632-69-9
Formula chimica: C20H2Cl4I4Na2O5
Codice SA: 38229000
Marca: Carl Roth
ROTI®Blue quick, 1x solution, ready-to-use, staining solution for protein gels, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C, UN-Nr. 1760


ROTI®Blue quick 1x conc., ready-to-use

ROTI®Blue quick provides a new, very fast staining method for proteins in polyacrylamide gels. Due to the modern formulation, the dye binds highly specifically to proteins, but not to the gel matrix, hence eliminating the need to destain the gel. First bands are clearly visible within 3 minutes!

  • Particularly fast, specific staining
  • First bands are visible within 3 mins.
  • No background - no destaining necessary
  • Very simple application without heating, methanol, or acetic acid
  • Stabilised for a longer shelf life
4829_GelB_Ausschnitt
Figure:
SDS-PAGE with 3 µl Roti®-Mark Standard and samples.
Staining duration: 20 mins.

Gebruiksinstructies

The following protein amounts / band can be detected in the specified time (minutes):
from 1 µg (3 min.)
from 100 µg (15 min.)
from 10 ng (60 min.)


The sensitivity of staining equals that of ROTI®Blue (Art. No. A152), so bands of 10 ng protein can be detected. ROTI®Blue quick is, therefore, approx. 5times more sensitive than standard Coomassie® staining solutions. ROTI®Blue quick staining is also fully compatible with usual down-stream applications like, membrane transfer & Western blotting, MS analysis (incl. MALDI) and protein gel elution.



Technische informatie
Sensitiviteit 10
Toepassing Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics.
Suitability of Protein Stainings

Product Sensitivity Rapid Gel

Analysis
Gel

Drying
Elution/

Transfer
2D-

Gels
MS-

Analysis
ROTI®Black P 1 ng/mm2 - +++ - +++ -
ROTI®Blue 10 ng/mm2 - +++ +++ - +++
ROTI®Blue quick 10 ng/mm2 +++ +++ ++ - ++
ROTI®Blau R 50 ng/mm2 +++ + - - -
+++: recommended +: suitable -: not recommended

Summary Staining of Protein Gels

Product Sensitivity Description
ROTI®Black P 1 ng/mm2 Silver staining kit. Highly sensitive. Simple, fast and reproducible.
ROTI®Blue 10 ng/mm2 Coomassie®-solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible.
ROTI®Blue quick 10 ng/mm2 Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics.
ROTIPHORESE®Blau R 50 ng/mm2 Coomassie® staining solution concentrate for rapid staining.
Coomassie® Blue G250 (C.I. 42655) 60 ng/mm2 Dye for performing Coomassie® staining. ODmax = 595
Coomassie® Blue R250 (C.I. 42660) 50 ng/mm2 Dye for performing Coomassie staining. ODmax = 550

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