20x stock solution sodium/sodiumcitrate for nucleic acid blotting.
Undiluted or after dilution with sterile water to 10x, 6x, 1x or 0,1x SSC one receives a versatile buffer for blotting, hybridising or washing of Southern- or Dot/Slot-Blots of DNA as well as for in situ hybridisation. Undiluted buffer is also suitable for neutral transfer of RNA onto unloaded membranes (e.g. ROTI®Nylon 0.2, Art. No. AE50.1).
Appearance of solution | water clear solution |
Colour | colorless |
pH value | 6.75-7.25 |
3.0 M NaCl, 300 mM sodium citrate in deionised water, pH-value 7.0, steam sterilised. |