ROTI®ZOL RNA is a ready-to-use, green-coloured solution for isolating total RNA from biological samples such as cells or tissue.
ROTI®ZOL RNA is based on the phenolic isolation procedure known from Chomczynski and Sacchi (Anal. Biochem. (1987) 162:156-9). Due to the advanced and optimized formulation, ROTI®ZOL RNA results in very high recovery rates of highly pure total RNA. Application is very convenient and preparation of several samples in parallel is finished in less than one hour. Additionally, the green colour significantly simplifies the separation of the RNA containing aqueous phase, the DNA containig interphase, and the protein containing phenolic phase during the process.
Typical RNA yield from 5x106 cells: 30-35 μg
Typical purity of RNA prepared from 5x106 cells: 2,08 (A260/A280)
Colour | green |
pH value (25 °C) | 4,1-4,7 |