Name: ROTIPHORESE®Sistema di sequenziamento del DNA
Brand: Carl Roth
SKU: A431.1
Price: 177.5 EUR
Availability: InStock

ROTIPHORESE®Sistema di sequenziamento del DNA

Dettagli del prodotto

Codice SA: 38229000

Marca: Carl Roth

ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, for DNA sequencing, Density (D) 1 g/cm³, Storage temp. +4 to +15 °C

For preparation of 4-20 % sequencing gels
Most simple handling of three optimised ready-to-use solutions
No tedious dissolving of urea
Prevents acrylamide dusts
Reproducible, superior quality of gels
In tried-and-tested ROTIPHORESE^® quality

Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE^® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.
In addition, the ROTIPHORESE^®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE^®Sequencing gel buffer concentrate and ROTIPHORESE^®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE^®10x TBE buffer or water, the urea content can be reduced to up to 40 %.

Gebruiksinstructies

Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (Art. no. AE28).

ROTIPHORESE^®DNA sequencing system ROTIPHORESE^® for DNA sequencing

The kit contains

1 l ROTIPHORESE^®sequencing gel concentrate (Art. No. 3043.1) - ready-to-use, stabilised solution of 25 % acrylamide/bisacrylamide 19:1 with 50 % urea (8,3 M).
1 l ROTIPHORESE^®sequencing gel diluent (Art. No. 3047.1) - 50 % (g/v; 8,3 M) solution of urea in water.
250 ml ROTIPHORESE^®sequencing gel buffer concentrate (Art. No. 3050.1) - 10x TBE with 50 % urea (8,3 M).
Detailed instructions for use are enclosed with the product.

Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE^® ready-to-use solution.

Superior quality
ROTIPHORESE^® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE^®-solutions, each batch undergoes electrophoretic testing.

The right choice for each and every application
The large variety of different ROTIPHORESE^® solutions provides you with the right product for each and every application. ROTIPHORESE^® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE^® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.

ROTIPHORESE^®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE^® ready-to-use solutions can be used directly.

Recommendation for Use of ROTIPHORESE^® Ready-Made Gel Solutions

Analysis of	Gel solution	Art. No.
Proteins/peptides >15 kDa, DNA fragments	Gel 30 (37,5:1)	3029.1
Proteins/peptides >15 kDa, DNA fragments	Gel 40 (37,5:1)	T802.1
Proteins/peptides <30 kDa and DNA oligonucleotides	Gel 40 (29:1)	A515.1
DNA sequences (manual sequencing)	ROTIPHORESE^®DNA sequencing system	A431.1
DNA sequences (manual sequencing)	Gel 40 (19:1)	3030.1
DNA sequences (short-run automat. sequencing)	NF solution 40 % (19:1)	A516.1
DNA sequences (long-run automat. sequencing)	NF solution 30 % (29:1)	A124.1
DNA sequences (long-run automat. sequencing)	NF solution 40 % (29:1)	A121.1

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