monoclonale murino anti-GAPDH, clone 6C5, 0,2 ml
Codice articolo 1NLP.1
€ 110,31
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monoclonale murino anti-GAPDH, clone 6C5, 0,2 ml
Dettagli del prodotto
Numero CAS: 72-19-5
Formula chimica: C4H9NO3
Codice SA: 30021500
Marca: PROGEN
anti-GAPDH mouse monoclonal,, clone 6C5, 100 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-GAPDH mouse monoclonal, clone 6C5 100 μg/ml, monoclonal, affinity purified
GAPDH (glyceraldehyde 3-phosphate dehydrogenase) is a 37 kDa enyzme that catalizes the conversion from glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate. Thereby, NADH is built. GAPDH is a commonly used as a loading control for Western botting or as a housekeeping gene standard in qPCR.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: 6C5
Immunogen: GAPDH purified from rabbit muscle
UniprotID: P62739 (bovine), P08023 (chicken), P62736 (human), P62737 (mouse), P62738 (rat)
Synonym: actin, aortic smooth muscle, alpha-actin-2, cell growth-inhibiting gene 46 protein [cleaved into: actin, aortic smooth muscle, intermediate form], ACTA2, ACTSA, ACTVS, GIG46
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: WB
Reactivity: human
Toepassing
Western Blot (WB): 1:5,000 (20 ng/ml)
Western blot analysis of HeLa lysate with anti-GAPDH antibody. Western blot analysis was performed on 20 μg HeLa lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-GAPDH antibody mouse monoclonal, clone 6C5 (Cat. No. 690975) was diluted in blocking buffer (antibody concentration 0.02 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.4 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
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