monoclonale murino anti-c-myc-tag, 9E10, 100 µg
Codice articolo 1NNT.2
€ 343,95
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monoclonale murino anti-c-myc-tag, 9E10, 100 µg
Dettagli del prodotto
Codice SA: 30021500
Marca: PROGEN
anti-c-myc-tag mouse, monoclonal, 9E10, after dissolv. in 400 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-c-myc-tag mouse monoclonal, 9E10 lyophilized, monoclonal, affinity purified
The monoclonal 9E10 antibody recognizes human c-myc, a 62 kDa transcription factor. A c-myc tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: 9E10
Immunogen: C-terminal peptide of human c-myc (aa 408-439)
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IP, WB
Reactivity: c-myc
Toepassing
Immunoprecipitation (IP): assay dependent
Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)
Western blot analysis of E. coli lysate containing c-myc-tagged protein with anti-c-myc-tag antibody. Western blot analysis was performed on 80 ng, 40 ng, 10 ng or 5 ng of E. coli lysate containing c-myc-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-c-myc-tag mouse monoclonal, 9E10 (Cat. No. 910MYCL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
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