monoclonale di topo anti-cheratina di tipo II, Ks pan1-8, 0,2 ml
Codice articolo 1NNH.1
€ 110,31
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monoclonale di topo anti-cheratina di tipo II, Ks pan1-8, 0,2 ml
Dettagli del prodotto
Codice SA: 30021500
Marca: PROGEN
anti-Keratin Type II mouse, monoclonal, Ks pan1-8, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Keratin Type II mouse monoclonal, Ks pan1-8 50 μg/ml, monoclonal, affinity purified
Ks pan1-8 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors.
Polypeptides reacting: Mr 52,500-Mr 68,000 keratins (type II keratins K1-K8; formerly also designated cytokeratins 1-8) of human epithelial cells.
Tumors specifically detected: all epithelium-derived neoplasms.
Reactivity on cultured cell lines MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12.
Host: mouse
Antibody type: monoclonal
Isotype: IgG2a
Clone: Ks pan1-8
Immunogen: cytoskeletal proteins from cultured human MCF-7 cells
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: amphibia, bovine, human, mouse, rat
Toepassing
Immunocytochemistry (ICC): Assay dependent
Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0.5-5 μg/ml, microwave treatment recommended)
Western Blot (WB): 1:500 (0.1 μg/ml)
Western blot analysis of HeLa lysate with anti-Keratin Type II antibody. Western blot analysis was performed on 20 μg HeLa lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Keratin TYpe II mouse monoclonal, Ks pan1-8 (Cat. No. 690006) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
IHC of human HNSCC tissue (courtesy of J.Heß, University Hospital Heidelberg)
IHC of mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)
Geen medisch hulpmiddel / Geen ivD-product
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