monoclonale murino anti-Strep-tag, C23.21, 100 µg
Codice articolo 1NLL.2
€ 343,95
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monoclonale murino anti-Strep-tag, C23.21, 100 µg
Dettagli del prodotto
Codice SA: 30021500
Marca: PROGEN
anti-Strep-tag mouse, monoclonal, , C23.21, after dissolv. in 1 ml PBS, 100 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Strep-tag mouse monoclonal, C23.21 lyophilized, monoclonal, affinity purified
The monoclonal C23.21 antibody recognizes Strep II. A Step-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1Clone: C23.21
Immunogen: Strep-tag II peptide minimal sequence WSHPQFEK
Synonym: ChR2
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: WB
Reactivity: Strep
Toepassing
Western Blot (WB): 1:5,000-1:10,000 (0.02-0.01 μg/ml)
Western blot analysis of E. coli lysate containing Strep-tagged protein with anti-Strep-tag antibody. Western blot analysis was performed on 80 ng, 40 ng, or 10 ng of E. coli lysate containing Strep-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Strep-tag mouse monoclonal, C23.21 (Cat. No. 910STRL) was diluted in blocking buffer (antibody concentration 0.02 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
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