monoclonale murino con tag anti-GFP, F56-6A1.2.3, 25 µg
Codice articolo 1NP5.1
€ 125,40
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monoclonale murino con tag anti-GFP, F56-6A1.2.3, 25 µg
Dettagli del prodotto
Codice SA: 30021500
Marca: PROGEN
anti-GFP-tag mouse, monoclonal, F56-6A1.2.3, after dissolv. in 100 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-GFP-tag mouse monoclonal, F56-6A1.2.3 lyophilized, monoclonal, affinity purified
The monoclonal F56-6A1.2.3 antibody recognizes the GFP-tag (green fluorescent tag). The GFP-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG2b
Clone: F56-6A1.2.3
Immunogen: full length enhanced GFP
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, WB
Reactivity: GFP
Toepassing
Immunocytochemistry (ICC): assay dependent
Western Blot (WB): 1:500 (0.5 μg/ml)
Western blot analysis of E. coli lysate containing GFP-tagged protein with anti-GFP-tag antibody. Western blot analysis was performed on 250 ng, 100 ng or 25 ng of E. coli lysate containing GFP-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-GFP-tag mouse monoclonal, F56-6A1.2.3 (Cat. No. 910GFPL) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
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