DAB Metal Enhancer

DAB metal enhancer is used to enhance the signal strength in peroxidase (HRP) detections and to modify the signal colour from brown to purplish gray. Applications include all immunochemical assays like immunohistochemistry and -cytochemistry as well as Western blotting, but DAB metal enhancer may also well be used during in situ hybridization.

When the DAB metal enhancer is applied, the sensitivity of DAB staining is about two times higher than with DAB alone.

For application, the DAB substrate components and buffer reagents (for instance ROTI^®DAB-Kit, Art. No. 9202) are simply diluted in DAB metal enhancer instead of water.
The purplish gray precipitate resulting from the staining reaction can be detected in visible light and does not bleach during long-term storage. Since it is insoluble in aqueous and organic solvents, slides may be permanently mounted in hydrophilic, or, after dehydration, in hydrophobic mounting medium.