HPLC column NUCLEODUR® 100-5 C18 ec 5 µm, 250 mm, 4 mm, 1 Piece(s)
SKU KK83.1
€ 494,61
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HPLC column NUCLEODUR® 100-5 C18 ec 5 µm, 250 mm, 4 mm, 1 Piece(s)
Product Details
HS code: 90279000
EAN: 8721028181831
Brand: Macherey-Nagel
Material: stainless steel.
The ideal phase for routine HPLC analysis.
Properties:
- Octadecyl phase, endcapped
- Carbon content: 17.5 %
- Pore size: 110 Å
- pH stability: 1-9
- Eluent on column: acetonitrile/water
Conditions:
Eluent: acetonitril/water (75/25, v/v); Flow: 1,3 ml/min; Temperature: 30 °C; Detection: UV, 254 nm
Legend:
1. Toluene, 2. Ethylbenzene, 3. Propylbenzene, 4. Butylbenzene, 5. Pentylbenzene
Eluent: acetonitril/water (75/25, v/v); Flow: 1,3 ml/min; Temperature: 30 °C; Detection: UV, 254 nm
Legend:
1. Toluene, 2. Ethylbenzene, 3. Propylbenzene, 4. Butylbenzene, 5. Pentylbenzene
Similar phases:
For many applicatons the following phases can be replaced with identical or even better results: NUCLEOSIL® C18, Spherisorb® ODS I und II, Hypersil™ ODS, Waters Symmetry®C18, Inertsil® ODS II, Kromasil® C18, LiChrospher® RP18 und RP C18 ec.
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.
Further HPLC columns from Macherey-Nagel available on request.
HPLC Columns NUCLEODUR® ec
Non-polar phases for routine analysis
Standard RP phase (110 Å) for daily routine analysis and up-scaling to preparative HPLC. Also available wide-pore (300 Å) for separation of biomolecules in analytical columns.
Properties:
- Cost-effective and reliable
- Long-life columns
- For all standard routine applications in modern RP-chromatography
Recommended application(s):
- Pore size 100 Å:
Basic, neutral and acidic pharmaceuticals, derivatised amino acids, pesticides, fat-soluble vitamins, aldehydes and ketones, phenolic compounds. - Pore size 300 Å:
Biological macromolecules, such as proteins or peptides. - USP L1 (C18) · USP L7 (C8) · USP L26 (C4)
HPLC column NUCLEODUR® 100-5 C18 ec 5 µm
Technical Information
| Particle size | 5 µm |
| Column length | 250 mm |
| Column inner diameter | 4 mm |
| Column volume | 3.15 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | 100-5 C18 ec |
| Surface chemistry | Medium density monomeric octadecyl (C18, ODS) modification |
| USP listing | L1 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 340 m²/g |
| Pore volume | 0.9 ml/g |
| Pore size | 110 Å |
| Temperature stability | 60 °C |
| Pressure stability | 600 bar |
| pH stability | 1,0-9,0 |
| Recommended application(s) | Aldehydes, Basic, neutral or acidic drugs, Derivatized amino acids, Fat-soluble vitamins, Ketones, Pesticides, Phenolic compounds |
| Similar phases | NUCLEOSIL® C18 Spherisorb® ODS I Spherisorb® ODS II Hypersil™ ODS Symmetry® C18 Inertsil® ODS II Kromasil® C18 LiChrospher® RP18 LiChrospher® RP C18 ec |
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
NUCLEODUR® Columns for HPLC
NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.
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