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HPLC column NUCLEODUR® 300-5 C4 ec, 150 mm, 4.6 mm, 1 Piece(s)

HPLC column NUCLEODUR® 300-5 C4 ec, 150 mm, 4.6 mm, 1 Piece(s)

SKU 1HKH.1
€ 429,07
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HPLC column NUCLEODUR® 300-5 C4 ec, 150 mm, 4.6 mm, 1 Piece(s)
Product Details
HS code: 90279000
Material: Stainless steel
EAN: 8721028181893
Brand: Macherey-Nagel
Material: stainless steel.

Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR® silica gel and is often used for the separation of biomolecules.


Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.

Properties:
  • Butyl phase, endcapped
  • Carbon content: 2.5 %
  • Pore size: 300 Å
  • pH stability: 1-9
  • Eluent on column: acetonitrile/water

Similar phases:
Jupiter® C4; ACE® C4.


Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.


Further HPLC columns from Macherey-Nagel available on request.


HPLC Columns NUCLEODUR® ec

Non-polar phases for routine analysis

Standard RP phase (110 Å) for daily routine analysis and up-scaling to preparative HPLC. Also available wide-pore (300 Å) for separation of biomolecules in analytical columns.

Properties:

  • Cost-effective and reliable
  • Long-life columns
  • For all standard routine applications in modern RP-chromatography

Recommended application(s):

  • Pore size 100 Å:
    Basic, neutral and acidic pharmaceuticals, derivatised amino acids, pesticides, fat-soluble vitamins, aldehydes and ketones, phenolic compounds.
  • Pore size 300 Å:
    Biological macromolecules, such as proteins or peptides.
  • USP L1 (C18) · USP L7 (C8) · USP L26 (C4)


HPLC column NUCLEODUR® 300-5 C4 ec

Technical Information
Particle size 5 µm
Column length 150 mm
Column inner diameter 4.6 mm
Column volume 2.5 ml
Endcapped yes
Eluent in column Acetonitrile - Water
Phase 300-5 C4 ec
Surface chemistry Medium density monomeric butyl (C4) wide-pore modification
USP listing L26
Separation principle Reversed phase (RP)
Column shape Analytical separation column
Column type HPLC column
Particle type Fully porous particles (FPP)
Specific surface according to BET 100 m²/g
Pore volume 0.9 ml/g
Pore size 300 Å
Temperature stability 60 °C
Pressure stability 450 bar
pH stability 1,0-9,0
Recommended application(s) Biological macromolecules, Peptides, Proteins
Similar phases Jupiter® C4
ACE® C4

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


NUCLEODUR® Columns for HPLC

NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.


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