Thin-layer chromatography (TLC, HPTLC) / HPTLC plates

Thin Layer Chromatography (DLC), also known as High Performance Thin Layer Chromatography (HPTLC), is an analytical technique used to separate and identify different components in a mixture. This technique uses thin layers of an adsorbent material, such as silica gel or aluminum oxide, applied to a glass or plastic plate. The principle of thin layer chromatography is based on the fact that different components in a mixture have different affinities for the adsorbent material. By applying the mixture to the thin layer and then placing the plate in a suitable solvent, the components will move along the plate based on their affinity. This creates a separation of the components, which can then be identified and quantified. One of the advantages of HPTLC is the high resolution that can be achieved. By using very fine particles of adsorbent material and an optimized mobile phase, very small differences in affinity between components can be detected. This makes it possible to separate and analyze complex mixtures with great accuracy. HPTLC plates are available in different sizes and with different adsorbent materials. The choice of plate depends on the specific application and the components to be analyzed. Silica gel plates are often used for general separations, while alumina plates are suitable for specific applications, such as the separation of polar compounds. In addition to the separation of components, HPTLC can also be used for the identification of unknown substances. By comparing the migration rates of unknown samples with those of known standards, the identity of the components can be determined. Furthermore, HPTLC can be coupled with other analytical techniques, such as mass spectrometry, to further improve the sensitivity and specificity of the analysis. In short, thin layer chromatography (DLC) or High Performance Thin Layer Chromatography (HPTLC) is a powerful analytical technique used to separate, identify and quantify components in a mixture. Using HPTLC plates and optimized separation techniques, complex mixtures can be analyzed with great accuracy. This technique is widely applicable in various industries, such as the pharmaceutical, food and environmental industries, and plays an important role in quality control and research.