Legionella Acid Buffer

To minimize the accompanying bacterial flora, 30 ±5 ml of Legionella Acid Buffer are applied to the membrane after membrane filtration. After an exposure time of approx. 5 minutes, the buffer is vacuumed off and the membrane rinsed with Pages Saline or a comparable buffer.
The filter is transferred sterile to a legionella selective agar, e.g. Legionella Agar (Base) (Art. No. CL48.1) and incubated.