ATP-dependent recombinant ligase for molecular cloning, site-directed mutagenesis, pitch repair in duplex DNA, RNA or DNA/RNA hybrids and ligation-mediated PCR.
T4 DNA Ligase catalyses the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl end groups in duplex DNA or RNA.
T4 DNA Ligase
Ligation buffer (10x)
ATP solution (25 mM)
The individual components of this kit cannot be purchased separately.The tubes included in the kit are colour coded to avoid confusion of reagents.
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Concentration | 5 U/µl |
DNA nucleases | not detectable |