Ligase Tth, 50 µl
SKU 3736.1
€ 125,40
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Ligase Tth, 50 µl
Product Details
Chemical formulas: C43H83NO8S
HS code: 35079090
Brand: Carl Roth
Ligase Tth, 5 U/µl, for Molecular Biology, Density (D) 1,18 g/cm³, Storage temp. -20 °C
Ligase Tth 5 U/µl, for molecular biology
Tth DNA Ligase catalyzes the NAD-dependent formation of phosphodiester bonds between adjacent 3'-hydroxyl and 5'-phosphate termini in double-stranded DNA. This ligase is inactive against single-stranded DNA or RNA and blunting DNA.
- stable at high temperatures
- ligates double-stranded DNA
- Repairs single strand breaks in double stranded DNA
The Tth DNA ligase is stable and active at much higher temperatures than conventional DNA ligases. The optimal ligation temperature range is 7-10 °C higher than that of the T4 DNA ligase and is determined by the Tm of the substrates. High ligation temperature eliminates the nonspecific ligation.
The kit contains:
Quick Ligase
Ligation buffer (10x)
The individual components of this kit cannot be purchased separately.
The tubes included in the kit are colour coded to avoid confusion of reagents.
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
| Concentration | 5 U/µl |
| DNA nucleases | not detectable |
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