Magnesium chloride solution, 1 ml, 2 x 0,5 ml in tubes

In a PCR reaction, cofactors such as divalent cations (MgCl₂) are needed for an optimal process. Magnesium interacts with the DNA template, dNTPs and the polymerase in a PCR reaction. The MgCl₂ concentration influences the productivity and accuracy of the polymerase. It also influences the binding of the primers to the template.