Transfer membrane ROTI®PVDF 0.45
SKU 200T.1
€ 548,69
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Transfer membrane ROTI®PVDF 0.45
Product Details
P-phrases: P210 P260 P280 P301+P310 P303+P361+P353 P305+P351+P338
H-phrases: H226-H304-H312+H332-H315-H319-H335-H373
CAS number: 1330-20-7
Chemical formulas: C8H10
Hazard class: 3 III
HS code: 39219090
Material: PVDF
Brand: Ratiolab GmbH
ROTI®PVDF, pore size 0.45 µm, Roll, 300 x 30 cm, PVDF Membrane
Materiaal: polyvinylidenfluoride (PVDF).
PVDF membrane for protein analyses.
ROTI®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.
Gebruiksinstructies
The membrane shows superior protein binding parameters and stands out for its particularly low background. It may also be used very well after repeated stripping and harsh washing processes.
This mechanically rigid transfer membrane can be easily cut to the desired gel size and it remains flexible and tear-proof even when dry.
Briefly immerse the membrane membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every blotting system.
Due to production processes, the membrane shows two different sides - one smooth and one raw. For optimally distinct bands, please use the smooth side for protein binding.
Transfer membrane ROTI®PVDF 0.45
Geschikt voor
Optimal for proteins≥20 kDa.
Detection by Chemoluminescence, radioactivity, colour.
Toepassingsgebieden:
- Western blotting
- Immuno-blotting
- Solid phase assays
- Amino acid or protein analysis
Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
| Afmeting | 300 × 30 cm |
| Poriëngr. | 0,45 µm |
| Binding | hydrophobic |
| Bindingscapaciteit | 125 µg/cm² |
| Dikte | 190±50 µm |
| Immobilisering | not necessary |
Technical Parameters of ROTH-Transfer Membranes
| Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
| Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
| Roti® PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 140 ±20 | ≥125 | 0,2 | not necessary |
| Roti® PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | ca. 200 | 0,45 | not necessary |
| Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
| Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
| Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
| Roti® NC | Nitrocellulose | electrostatic, hydrophobic | 150 ±50 | 125-200 | 0,2 | UV, baking (max. 80 °C) |
| Roti® NC 0.45 | Nitrocellulose | electrostatic, hydrophobic | 150 ±10 | ≥125 | 0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
Size Comparison of Membrane Pores and Biomolecules
| Biomolecules / Membrane Pores | Size (kD) |
| genomic DNA (human) | >11000000 |
| E. coli | approx. 1500000 |
| 0.45 μm | approx. 5000 |
| 0.2 μm | approx. 2000 |
| DNA 3 kb | approx. 1000 |
| RNA 1 kb | approx. 300 |
| Antibodies (IgG) | approx. 150 |
| Proteins in the lab | approx. 10–300 |
| BSA (Albumin) | 69 |
Application of ROTH-Transfer Membranes
| Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
| DNA | +++ | + | - | - | - | + | + |
| RNA | + | +++ | - | - | - | + | + |
| Proteins | - | + | +++ | +++ | +++ | + | + |
| Detection System | |||||||
| Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
| Fluorescence | +++ | +++ | - | - | +++ | - | - |
| +++: recommended | +: suitable | -: not | |||||
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